Table 2

 Primer sequences and temperatures for touchdown protocols

SenseAntisenseT2° target TStep
T, temperature; TNF-α, tumour necrosis factor α; IL-8, interleukin 8; HD-5, human defensin 5; HD-6, human defensin 6; sPLA2, secretory phospholipase A2; HPRT, human hypoxanthine phosphoribosyltransferase.
TNF-α5′TCT GGC CCA GGC AGT CAG ATC 3′5′TCA GCT TGA GGG TTT GCT ACA A 3′65°C59°C1°C
IL-85′ATG ACT TCC AAG CTG GCC GTG GC 3′5′TCT CAG CCC TCT TCA AAA ACT TC 3′66°C60°C1°C
HD-55′ GCC ATC CTT GCT GCC ATT C 3′5′ AGA TTT CAC ACA CCC CGG AGA 3′66°C60°C1°C
HD-65′ CCT CAC CAT CCT CAC TGC TGT TC 3′5′ CCA TGA CAG TGC AGG TCC CAT A 3′66°C60°C1°C
Lysozyme5′AAA ACC CCA GGA GCA GTT AAT 3′5′CAA CCC TCT TTG CAC AAG CT 3′60°C
sPLA25′TGA CGA CAG GAA AGG AAG CCG CAC 3′5′AGG GAA GAG GGG ACT CAG CAA CGA G 3′60°C
HPRT5′ATC AGA CTG AAG AGC TAT TGT AAT GAC CA 3′5′ TGG CTT ATA TCC AAC ACT TCG TG 3′66°C60°C1°C