Effects of interleukin (IL)-1β and Helicobacter pylori on matrix metalloproteinase 3 (MMP-3) activity of AGS cell conditioned media
| Sample | Initial reaction velocity (% of AGS) |
|---|---|
| Hydrolysis of M2110, an MMP-3 specific synthetic fluorogenic susbstrate, by concentrated serum free culture media of IL-1β treated (0.2–20 ng/ml) and/or H pylori infected (MOI=50) AGS cells and bacterial supernatant alone (Hp). | |
| Data are expressed as a percentage of the initial reaction velocity of MMP-3 activity in untreated AGS cell conditioned medium (mean (SEM)). | |
| Medium refers to cell culture medium alone and AGS refers to untreated/uninfected AGS cell conditioned medium (n=3). | |
| MMP-3 activity of every sample was significantly different from AGS (**p<0.01). Interaction between IL-1β and H pylori, as calculated by two way ANOVA, was p<0.001. | |
| AGS | 100 (5) |
| AGS+IL-1β (0.2 ng/ml) | 144 (26)** |
| AGS+IL-1β (2 ng/ml) | 149 (20)** |
| AGS+IL-1β (20 ng/ml) | 199 (11)** |
| AGS+Hp | 2375 (11)** |
| AGS+Hp+IL-1β (0.2 ng/ml) | 2042 (12)** |
| AGS+Hp+IL-1β (2 ng/ml) | 1662 (6)** |
| AGS+Hp+IL-β (20 ng/ml) | 1944 (7)** |
| Helicobacter pylori | 291 (39)** |
| Medium | 11 (8)** |