Control Isc response (μA/cm2) | Isc response in the presence of barium (μA/cm2) | Isc response in the presence of charybdotoxin+apamin (μA/cm2) | |
---|---|---|---|
Barium (5 mM) and charybdotoxin (0.5 μM) plus apamin (0.5 μM) were given 20 minutes before secretogogues to the basolateral surface. Acetylcholine (ACh) was given 180 minutes after cholera toxin (CT) and five minutes after forskolin. | |||
Data are means (SEM) (n = 6). | |||
*p<0.05 compared with controls. | |||
ND, no data. | |||
CT 0.01 μg/ml (n = 5) | 34 (2) | 12 (1)* | ND |
ACh 10 μM (n = 5) | 10 (3) | 12 (1) | ND |
ACh 10 μM (180 min after CT 0.01 μg/ml; n = 5) | 133 (8) | 137 (26) | ND |
Forskolin 10 μM (n = 6) | 22 (1) | 11 (1)* | 27 (2) |
ACh 50 μM (5 min after forskolin 10 μM; n = 6) | 112 (9) | ND | 41 (3)* |