Wild-type | Wild-type Anti-Fas | Wild-type AntiFas+adv-IκBα | IL-18 Tg | IL-18 Tg Anti-Fas | IL-18 Tg Anti-Fas+adv-IκBα | |
---|---|---|---|---|---|---|
Hepatocytes from wild-type and interleukin 18 transgenic (IL-18 Tg) mice were isolated followed by Fas cross linking and adenoviral (adv) gene transfer of IκB, as indicated. Annexin V/propidium iodide (PI) staining was performed, as described in the methods section. | ||||||
Results from two representative experiments are reported as per cent of apoptotic cells. | ||||||
Experiment 1 | 35.69 | 54 | 49.38 | 75.25 | 75 | 72.37 |
Experiment 2 | 35.69 | 40.45 | 75.25 | 74.93 | ||
AnnexinV+/PI+ | ||||||
Experiment 1 | 16.95 | 29.97 | 33.06 | 12.24 | 14.39 | 16.85 |
Experiment 2 | 16 | 24.10 | 12.5 | 11.5 | ||
AnnexinV+/PI− |