Primers and restriction enzymes used for caspase recruitment domain 15 (CARD15) and Toll-like receptor 4 (TLR4) genotyping
| Gene | Variant | Primers | Restriction enzyme |
|---|---|---|---|
| CARD15 | Arg702Trp | 5′-AGATCACAGCAGCCTTCCTG-3′ | MSPI |
| 5′-CACGCTCTTGGCCTCACC-3″ | |||
| CARD15 | Gly908Arg | 5′-CCCAGCTCCTCCCTCTTC-3′ | HhaI |
| 5′-AAGTCTGTAATGTAAAGCCAC-3′ | |||
| CARD15 | Leu1007InsC | 5′-GGCAGAAGCCCTCCTGCAGGGCC-3′ | ApaI |
| 5′-CCTCAAAATTCTGCCATTCC-3′ | |||
| TLR4 | Asp299Gly | 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′ | NCoI |
| 5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′ |