Table 1

 Oligonucleotide primer sequences and polymerase chain reaction (PCR) conditions

mRNASense and antisense primersExpected size (bp)PCR conditions (cycles)
MCP-1, monocyte chemoattractant protein 1; TGF-β, transforming growth factor β; PDGF, platelet derived growth factor; IL-1β, interleukin 1β; IL-6, interleukin 6; mMCP-1, mutant MCP-1; GAPDH, glyceraldehyde phosphate dehydrogenase.
MCP-1Sense5′-ACTATGCAGGTCTCTGTCACG-3′41794°C, 1′; 59°C, 1′; 72°C, 1′ (30)
Antisense5′-AAGTGCTTGAGGTGGTTGTGG-3′
TGF-βSense5′-CTTCAGCTCCACAGAGAAGAACTGC-3′28991°C, 1′; 56°C, 1′; 72°C, 2′ (32)
Antisense5′-CACGATCATGTTGGACAACTGCTCC-3′
PDGF-BBSense5′-CAAGCCAGTCTTCAAGAAGGCCAC-3′14894°C, 30″; 65°C, 30″; 72°C, 1′ (34)
Antisense5′-AACGGTCACCCGAGTTTGAGGTGT-3′
IL-1βSense5′-GTGTGATGTTCCCATTAGACAGC-3′38394°C, 1′; 59°C, 1′; 72°C, 1′ (33)
Antisense5′-TGAGAGGTGCTGATGTACCAGTT-3′
IL-6Sense5′-CCTTCCCTACTTCACAAGTC-3′50194°C, 1′; 56°C, 1′; 72°C, 1′ (34)
Antisense5′-GGATGGTCTTGGTCCTTAGC-3′
mMCP-1Sense5′-ATGAAAGTCTCTGCCGCCCTT-3′28194°C, 1′; 60°C, 1′; 72°C, 1′ (35)
Antisense5′-TCAAGTCTTCGGAGTTTGGGT-3′
GAPDHSense5′-TCCATGACAACTTTGGCATCGTGG-3′37794°C, 30″; 60°C, 1′; 72°C, 1′ (30)
Antisense5′-GTTGCTGTTGAAGTCACAGGAGAC-3′