Effect of intracisternal corticotropin releasing factor (CRF) on mean arterial pressure in urethane anaesthetised rats
| Time after intracisternal injection (minutes) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 15 | 30 | 45 | 60 | 75 | 90 | 105 | 120 | |
| Values are mean (SEM) mean arterial pressure (mm Hg), five rats per group. | |||||||||
| After the basal observation, saline vehicle, CRF (2 nmol), or urocortin II (50 pmol) was injected intracisternally. Systemic arterial blood pressure was monitored thereafter for 120 minutes. Hepatic branch vagotomy was performed 120 minutes before, atropine methyl nitrate (0.15 mg/kg intraperitoneally) was injected 15 minutes before, hepatic plexus denervation by 85% phenol was performed seven days before, and 6-hydroxydopamine was administered seven days before (100 mg/kg) and four days before (80 mg/kg) intracisternal injection of peptide. | |||||||||
| *p<0.05 compared with the respective basal period. | |||||||||
| No treatment | |||||||||
| Saline | 101 (3) | 99 (7) | 102 (5) | 100 (6) | 102 (5) | 99 (3) | 101 (2) | 101 (3) | 101 (2) |
| CRF | 98 (4) | 105 (4)* | 108 (5)* | 111 (4)* | 107 (4)* | 106 (5)* | 105 (4)* | 102 (7) | 99 (6) |
| Urocortin II | 101 (4) | 99 (3) | 102 (3) | 100 (2) | 102 (2) | 99 (4) | 102 (3) | 99 (4) | 102 (5) |
| Vagotomy | |||||||||
| Saline | 102 (3) | 98 (3) | 99 (4) | 101 (3) | 101 (2) | 101 (3) | 99 (4) | 98 (4) | 101 (3) |
| CRF | 99 (4) | 106 (3)* | 109 (4)* | 112 (6)* | 109 (4)* | 107 (3)* | 106 (3)* | 101 (3) | 98 (2) |
| Atropine | |||||||||
| Saline | 98 (3) | 101 (3) | 101 (4) | 101 (4) | 102 (4) | 98 (3) | 101 (2) | 99 (4) | 100 (3) |
| CRF | 101 (4) | 106 (4)* | 110 (6)* | 109 (4)* | 108 (5)* | 108 (4)* | 107 (4)* | 104 (4) | 102 (2) |
| Hepatic plexus denervation | |||||||||
| Saline | 101 (2) | 102 (3) | 99 (5) | 102 (3) | 101 (3) | 98 (5) | 99 (3) | 102 (4) | 99 (5) |
| CRF | 99 (4) | 104 (2)* | 109 (4)* | 108 (4)* | 110 (5)* | 107 (4)* | 106 (4) | 101 (5) | 99 (3) |
| 6-hydroxydopamine | |||||||||
| Saline | 72 (4) | 70 (4) | 68 (3) | 70 (4) | 72 (3) | 69 (4) | 68 (4) | 70 (3) | 70 (2) |
| CRF | 69 (3) | 70 (3) | 69 (5) | 71 (3) | 69 (3) | 68 (3) | 69 (5) | 71 (4) | 72 (4) |