Table 1

 Polymerase chain reaction primers and annealing temperatures used

GenePrimerAnnealing temp (°C)Size (bp)Reference
IGF-I, IGF-II, insulin-like growth factors I and II; IGF-Ir, insulin-like growth factor I receptor; IGF-2r, insulin-like growth factor receptor 2.
IGF-I5′-CACTGTCACTGCTAAATTCA-3′60396Hana27
5′-CTGTGGGCTTGTTGAAATAA-3′
IGF-II5′-AGTCGATGCTGGTGCTTCTCA-3′60468Quinn28
5′-GTGGGCGGGGTCTTGGGTGGGTAG-3′
IGF-Ir5′-ATTGAGGAGGTCACAGAGAAC-3′60755Quinn28
5′-TTCATATCCTGTTTTGGCCTG-3′
IGF-2r5′-TCAACATCTGTGGAAGTGTG-3′62430Bredin29
3′-GAATAGAGAAGTGTCCGGATCGGAGTC-5′