Fold induction of RegIII and interferon γ (IFN-γ) inducible genes in the colon of either altered Schaedler’s flora (ASF) or segmented filamentous bacteria (SFB) colonised C.B17.SCID mice relative to those housed in a germ free environment
| Gene | ASF | SFB |
|---|---|---|
| Total RNA isolated from the colon of SCID mice housed in the designated environments was analysed using the Affymetrix MG_U74A microarray. After employment of standard normalisations, the average gene expression values between conditions were used to determine fold induction (n = 3 for each condition). | ||
| Upase, uridine phosphorylase. | ||
| RegIIIβ | 11.48 | 9.82 |
| RegIIIγ | 8.64 | 8.64 |
| IRG 47 | 1.84 | 2.10 |
| Rab6 | 2.43 | 2.15 |
| UPase | 5.64 | 1.79 |
| Guanylate nucleotide binding protein | 2.47 | 1.41 |