Table 1

 Sequences of short interfering RNAs (siRNAs)

siRNASequenceComment
Starting position indicates position of the first nt of the siRNA sense strand in the bcl-2 mRNA (accession No M13994). Specificity of the sequences was verified by a BLAST search against human and murine databases to minimise unspecific or off target effects.
Bcl15′-CAGGACCUCGCCGCUGCAGACC-3′Starting position: 1659
3′-CGGUCCUGGAGCGGCGACGUCUGG-5′
Bcl35′-GCCUUUGUGGAACUGUACGGCC-3′Starting position: 2047
3′-UACGGAAACACCUUGACAUGCCGG-5′
Bcl55′-CAGGACCUCGCCGCUGCAGAC-3′Starting position: 1659
3′-CGGUCCUGGAGCGGCGACGUCUG-5′
Bcl85′-GCCUUUGUGGAACUGUACGGC-3′Starting position: 2047
3′-UACGGAAACACCUUGACAUGCCG-5′
Bcl115′-UGUGGAUGACUGAGUACCUGA-3′Starting position: 1982
3′-GGACACCUACUGACUCAUGGACU-5′
Control15′-GAUGAGGAUCGUUUCGCAUGA-3′Eukaryotic kanamycin resistance protein
3′-UCCUACUCCUAGCAAAGCGUACU-5′
Control25′-CCACAUGAAGCAGCACGACUU-3′EGFP expression vector
3′-CUGGUGUACUUCGUCGUGCUGAA-5′
Control35′-ACGGCUAGCUGUGAAAGGUCC-3′3′ UTR of hepatitis C virus
3′-UCUGCCGAUCGACACUUUCCAGG-5′