Expression of ephrin-A1 induced genes in 11 α-fetoprotein (AFP) overexpressing hepatocellular carcinomas
| Case | AFP RIA* (ng/gTP) | AFP † | Ephrin-A1† | EphA1† | EphA2 † | p21† | TSP-1† | MMP-2† |
|---|---|---|---|---|---|---|---|---|
| *AFP RIA(ng/gTP) indicates the quantity of α-fetoprotein per g total protein after quantification of AFP expression by the radioimmunoassay method. | ||||||||
| †Relative amounts of AFP, ephrin-A1, EphA1, EphA2, p21, TSP-1, and MMP-2 mRNA fragments by northern blotting were measured by densitometry and normalised to the level of β-actin. The ratios of the cancerous mRNA expression level to non-cancerous mRNA expression level (T/N 18 ratios) are indicated (T/N ratio <0.50, − −; 0.50 <T/N ratio <0.67, −; 0.67 <T/N ratio <1.50, +/−; 1.50< T/N ratio <2.00, +; 2.00 <T/N ratio, ++). | ||||||||
| 3 | 612 | ++ | + | ++ | − − | +/− | − − | ++ |
| 4 | 2454 | ++ | ++ | ++ | − − | − − | − − | ++ |
| 5 | 185 | ++ | ++ | ++ | − − | − | +/− | ++ |
| 6 | 2776 | ++ | + | ++ | − − | − − | − − | ++ |
| 7 | 2816 | ++ | ++ | ++ | +/− | − − | − − | ++ |
| 9 | 3613 | ++ | ++ | ++ | − − | +/− | − − | ++ |
| 11 | 1526 | ++ | ++ | ++ | − − | − | − | − |
| 12 | 1684 | ++ | ++ | ++ | − − | − − | − − | + |
| 15 | 4671 | ++ | ++ | ++ | − − | +/− | − | ++ |
| 16 | 1339 | ++ | ++ | ++ | − − | − − | +/− | ++ |
| 18 | 1311 | ++ | ++ | ++ | − − | − − | +/− | + |