Polymerase chain reaction primers used
| Target bacteria | Primer | Sequence (5′→3′) | Product size (bp) | Reference |
|---|---|---|---|---|
| Prevotella | g-Prevo-F | CACRGTAAACGATGGATGCC | 527–529 | Matsuki et al27 |
| g-Prevo-R | GGTCGGGTTGCAGACC | |||
| Bifidobacterium spp | g-Bifid-F | CTCCTGGAAACGGGTGG | 549–563 | Matsuki et al27 |
| g-Bifid-R | GGTGTTCTTCCCGATATCTACA | |||
| Clostridium coccoides group | g-Ccoc-F | AAATGACGGTACCTGACTAA | 438–441 | Matsuki et al27 |
| g-Ccoc-R | CTTTGAGTTTCATTCTTGCGAA | |||
| Clostridium clostridiiforme | CC-1 | CCGCATGGCAGTGTGTGAAA | 255 | Wang et al26 |
| CC-2 | CTGCTGATAGAGCTTTACATA | |||
| Bacteroides fragilis group | g-BfraF | ATAGCCTTTCGAAAGRAAGAT | 501 | Matsuki et al27 |
| g-BfraR | CCAGTATCAACTGCAATTTTA | |||
| Bacteroides thetaiotaomicron | BT-1 | GGCAGCATTTCAGTTTGCTTG | 423 | Wang et al26 |
| BT-2 | GGTACATACAAAATTCCACACGT | |||
| Bacteroides vulgatus | BV-1 | GCATCATGAGTCCGCATGTTC | 287 | Wang et al26 |
| BV-2 | TCCATACCCGACTTTATTCCTT | |||
| Bacteroides distasonis | BD-1 | GTCGGACTAATACCGCATGAA | 273 | Wang et al26 |
| BD-2 | TTACGATCCATAGAACCTTCAT | |||
| Bifidobacterium adolescentis | BIA-1 | GGAAAGATTCTATCGGTATGG | 244 | Wang et al26 |
| BIA-2 | CTCCCAGTCAAAAGCGGTT | |||
| Bifidobacterium longum | BIL-1 | GTTCCCGACGGTCGTAGAG | 153 | Wang et al26 |
| BIL-2 | GTGAGTTCCCGGCATAATCC | |||
| Eubacterium biforme | EBI-1 | GCTAAGGCCATGAACATGGA | 46 | Wang et al26 |
| EBI-2 | GCCGTCCTCTTCTGTTCTC | |||
| Fusobacterium prausnitzii | FPR-1 | AGATGGCCTCGCGTCCGA | 199 | Wang et al26 |
| FPR-2 | CCGAAGACCTTCTTCCTCC | |||
| Peptostreptococcus productus | PSP-1 | AACTCCGGTGGTATCAGATG | 268 | Wang et al26 |
| PSP-2 | GGGGCTTCTGAGTCAGGTA | |||
| Lactobacillus acidophilus | LAA-1 | CATCCAGTGCAAACCTAAGAG | 286 | Wang et al26 |
| LAA-2 | GATCCGCTTGCCTTCGCA | |||