Primers and probes used in this study
| Primer or probe | 5′ to 3′ sequence | Experiments |
|---|---|---|
| Probes Clep 866, Erec482, and Bac 303 were developed to detect members of the Clostridium leptum, Clostridium coccoides, and Bacteroides groups, respectively. ACM 008 F and EUB 338 were used as primers to develop a probe for 16S rDNA library hybridisation after polymerase chain reaction amplification, using DNA extracted from faecal samples and [α-33P]dATP labelling. | ||
| *Personal communication from D Le Paslier. | ||
| †Lay et al, 2005.22 | ||
| F, forward; R, reverse. FISH, fluorescent in situ hybridisation. | ||
| ACM 008 F* | AGAGTTTGATCCTGGCTCAG | Sequencing |
| Macroarray hybridisation | ||
| ADM 330 F* | ACTCCTACGGGAGGCAGC | Sequencing |
| SSM 1100 R* | GGGCCTTGTACACACCG | Sequencing |
| TTM 1517 R* | GYAACGAGCGCAACCC | Sequencing |
| NON 338† | ACATCCTACGGGAGGC | FISH |
| EUB 338† | GCTGCCTCCCGTAGGAGT | FISH |
| Macroarray hybridisation | ||
| Clep 866† | GGTGGATWACTTATTGTG | FISH |
| Clep 866 competitor 1† | GGTGGAWACTTATTGTG | FISH |
| Clep 866 competitor 2† | GGTGGATWACTTATTGCG | FISH |
| Bac 303† | CCAATGTGGGGACCTT | FISH |
| Erec 482† | GCTTCTTAGTCARGTACCG | FISH |