Primer sequences and polymerase chain reaction conditions for the cathepsin B gene
| Region† | Name | Sequence (5′-3′) | Tann (°C) |
|---|---|---|---|
| F, forward; R, reverse; Tann, annealing temperature; *with DMSO. | |||
| †As per NCBI sequence Accession No NT_077531 and transcript id = “NM_147782.1”. | |||
| ‡Region from exon 1 to 2 forms part of the 5′ untranslated region. | |||
| Exon 1‡ | 1F | gcg gcg gaa ggt ggc ggg ag | 62* |
| 1R | cgc gga ctc cca ccc cag cc | ||
| Exon 2‡ | 2F | gcc tta tcg tcc gcc tct gt | 66 |
| 2R | gtg gga gga ggc aag ggg at | ||
| Exon 3 and 4 | 3F | aga cgg tgc ccc tgt gtg tg | 61 |
| 4R | ggc ctt cac tct ccc act tc | ||
| Exon 5 | 5F | tgt gct cat ttc ctt gtt ag | 51 |
| 5R | aaa aga aga ttg cta aga tta t | ||
| Exon 6 | 6F | gcg tcc cct ggt gtt gag ag | 63 |
| 6R | gcc cca ccc cta ccc aca aa | ||
| Exon 7, 8 and 9 | 7F | tgg tct gga gag ctg gtg gt | 64 |
| 8F | tgc ccc agg tct tct ccg tg | ||
| 8R | gcc ctg acc tct tcg ctg ca | ||
| 9R | ccc tct tcc cca gcc cct ca | ||
| Exon 10 and 11 | 10F | ggg gtg ctg tgg ggc gtg g | 61 |
| 11R | ggg gaa gga cgc tct gtg ct | ||