Table 1

 Antibodies used in the study

AntibodySourceDilution
IHCICCWestern
AP, alkaline phosphatase; HRP, horseradish peroxidase; ICC, immunocytochemistry; IHC, immunohistochemistry; Western, western blotting.
Primary rabbit polyclonal antibodies
    Anti-turkey syneminR Robson1:400
    Anti-human syneminZ Li1:8001:8001:1000
    Anti-vinculin (H-300) sc-5573Santa Cruz1:5001:1000
Primary mouse monoclonal antibodies
    Anti-porcine desmin clone DE-U-10 (IgG1)Sigma1:3001:5001:1000
    Anti-human vimentin clone VIM3.2 (IgM)Sigma1:5001:5001:1000
    Anti-porcine GFAP (IgG1) clone G-A-5Sigma1:20001:5001:1000
    Anti-α-smooth muscle actin (IgG2a) clone 1A4Sigma1:2501:1000
    Anti-β-actin clone AC-15 (IgG2a)Sigma1:2000
    Anti-vinculin clone hVIN-1 (IgG1)Sigma1:20001:1000
    Anti-talin clone 8D4 (IgG1)Sigma1:2501:1000
    Anti-α-actinin (IgM) clone BM-75.2Sigma1:5001:5001:1000
    Anti-paxillin (IgG1) clone 5H11Upstate1:250
    Anti-plectin (IgG1) clone 7A8Sigma1:200
Secondary antibodies
    Anti-mouse antibody HRP conjugatedAmersham1:5001:2000
    Anti-mouse antibody AP conjugatedAmersham1:5001:2000
    Anti-rabbit antibody HRP conjugatedAmersham1:5001:2000
    Anti-mouse IgG antibody Alexa Flour 488Molecular Probes1:200
    Anti-mouse IgG antibody Alexa Flour 633Molecular Probes1:200
    Anti-mouse IgG1 antibody Alexa Flour 488Molecular Probes1:200
    Anti-mouse IgG2a antibody Alexa Flour 633Molecular Probes1:200
    Anti-rabbit IgG antibody Alexa Flour 488Molecular Probes1:200
    Anti-rabbit IgG antibody Alexa Flour 633Molecular Probes1:200