Primer pairs used for PCR reactions to detect hepatocyte-, adipocyte- and osteocyte-specific gene expression in differentiated hBM-MSCs
| Gene of interest | Primer pair | Product length (base pairs) | Annealing temperature (°C) |
|---|---|---|---|
| CK 18 | 5′-GAAGGAGACCATGCAAAGCCTG-3′ | 400 | 62 |
| 5′-CATGAAGAGCAGCTCCTCCTTG-3′ | |||
| CK 19 | 5′-CGAACCAAGTTTGAGACGGAAC-3′ | 600 | 62 |
| 5′-CCGCTGGTACTCCTGATTCTGC-3′ | |||
| CK 7 | 5′-TCATCGACAAGGTGCGGTTTC-3′ | 400 | 62 |
| 5′-CTGCAGCTCTGTCAACTCCGTC-3′ | |||
| CX 32 | 5′-GGCGTGAACCGGCATTCTAC-3′ | 400 | 61 |
| 5′-ACAACAGCCGGAACACCACG-3′ | |||
| CX 43 | 5′-TGGCCTTCTTGCTGATCCAG-3′ | 400 | 58 |
| 5′-TTGCGGCAAGAAGAATTGTTTC-3′ | |||
| AFP | 5′-TTGCCCAGTTTGTTCAAGAAGC-3′ | 400 | 59 |
| 5′-CGAGCAGCCCAAAGAAGAATTG-3′ | |||
| Transferrin | 5′-GTGGCCTTTGTCAAGCA-3′ | 565 | 52 |
| 5′-CTCCATCCAAGCTCATG-3′ | |||
| Albumin | 5′-GATGTCTTCCTGGGCA-3′ | 645 | 52 |
| 5′-CTTGGGCTTGTGTTTCAC-3′ | |||
| PCK1 | 5′-GGCCGCACCATGTATG-3′ | 398 | 58 |
| 5′-AGGATCAGCATGTGCTC-3′ | |||
| CPS | 5′-CTGTAAAAGCCATGAAGG-3′ | 399 | 60 |
| 5′-CAATGGTGTCTGCTGCC-3′ | |||
| CYP3A4 | 5′-TCTCATCCCAGACTTGGCCAT-3′ | 300 | 55 |
| 5′-GGAGACAGAATAACATTCTT-3′ | |||
| GAPDH | 5′-CCA TGG AGA AGG CTG GGG-3′ | 195 | 63 |
| 5′-CAA AGT TGT CAT GGA TGA CC | |||
| LPL | 5′-ATG GAG AGC AAA GCC CTG CTC-3′ | 299 | 63 |
| 5′-TAC AGG GCG GCC ACA AGT TTT-3′ | |||
| PPAR-γ2 | 5′-GCT GTT ATG GGT GAA ACT CTG-3′ | 351 | 63 |
| 5′-ATA AGG TGG AGA TGC AGG CTC-3′ | |||
| Osteocalcin | 5′-GTG CAG AGT CCA GCA AAG GT-3′ | 274 | 60 |
| 5′-CTG GAG AGG AGC AGA ACT GG-3′ | |||
| Osteonectin | 5′-GTG CAG AGG AAA CCG AAG AG-3′ | 202 | 63 |
| 5′-AAG TGG CAG GAA GAG TCG AA-3′ |