Table 1 Specifications of antibodies, with their host, dilution and provider
AntigenDilutionSource
BDNF, rabbit1:600Santa Cruz Biotechnologies, Santa Cruz, CA
TrkB, rabbit1:600Santa Cruz Biotechnologies, Santa Cruz, CA
Synaptophysin, rabbit1:1000Dr R. Jahn, Göttingen, Germany
PGP9.5, mouse1:300UltraClone Ltd, Cambridge, UK
ChAT, mouse1:250Chemicon International, Temecula, CA
nNOS, mouse1:200BD Transduction Laboratories, San Diego, CA
5-HT, mouse1:20DakoCytomation A/S, Glostrup, Denmark
Mouse-IgG Alexa 594, donkey1:1000Molecular Probes, Leiden, The Netherlands
Rabbit-IgG Alexa 488, donkey1:1000Molecular Probes, Leiden, The Netherlands
Rabbit-IgG AMCA, donkey1:250Jackson Immuno, West Grove, PA
  • AMCA, aminomethylcoumarin acetate; BDNF, brain-derived neurotrophic factor; ChAT, choline acetyltransferase; 5-HT, 5-hydroxytryptamine; nNOS, neuronal nitric oxide synthase; PGP9.5, protein gene product 9.5; TrkB,tropomyosin-related kinase B.