Antigen | Dilution | Source |
BDNF, rabbit | 1:600 | Santa Cruz Biotechnologies, Santa Cruz, CA |
TrkB, rabbit | 1:600 | Santa Cruz Biotechnologies, Santa Cruz, CA |
Synaptophysin, rabbit | 1:1000 | Dr R. Jahn, Göttingen, Germany |
PGP9.5, mouse | 1:300 | UltraClone Ltd, Cambridge, UK |
ChAT, mouse | 1:250 | Chemicon International, Temecula, CA |
nNOS, mouse | 1:200 | BD Transduction Laboratories, San Diego, CA |
5-HT, mouse | 1:20 | DakoCytomation A/S, Glostrup, Denmark |
Mouse-IgG Alexa 594, donkey | 1:1000 | Molecular Probes, Leiden, The Netherlands |
Rabbit-IgG Alexa 488, donkey | 1:1000 | Molecular Probes, Leiden, The Netherlands |
Rabbit-IgG AMCA, donkey | 1:250 | Jackson Immuno, West Grove, PA |
AMCA, aminomethylcoumarin acetate; BDNF, brain-derived neurotrophic factor; ChAT, choline acetyltransferase; 5-HT, 5-hydroxytryptamine; nNOS, neuronal nitric oxide synthase; PGP9.5, protein gene product 9.5; TrkB,tropomyosin-related kinase B.