Table 4 Expression fold changes and range of expression levels (log₂ ratio) determined by expression microarray and by qRT-PCR, comparing either carcinomas vs adenomas (Ca/Ad) or tumours with 20q gain vs tumours without 20q gain (20q gain/non-20q gain)

Gene	Comparison	Microarray fold change	qRT-PCR fold change	Microarray expression range*	qRT-PCR expression range*
C20orf24	Ca/Ad	1.54	1.78	(−0.45 to 1.60)/(−0.71 to 0.71)	(1.84 to 6.08)/(−0.26 to 4.81)
	20q gain/non-20q gain	1.68	3.99	(−0.17 to 1.60)/(−0.71 to 0.37)	(−0.26 to 6.08)/(1.85 to 4.95)
AURKA	Ca/Ad	1.91	3.39	(−2.01 to 0.17)/(−2.26 to −1.11)	(−1.78 to 6.06)/(−0.64 to 3.72)
	20q gain/non-20q gain	1.55	4.53	(−2.11 to 0.17)/(−2.26 to −0.48)	(1.03 to 6.06)/(−1.78 to 3.99)
RNPC1	Ca/Ad	1.74	nd	(−1.61 to 1.22)/(−1.80 to −0.41)	nd
	20q gain/non-20q gain	1.58	nd	−1.71 to –1.22/−1.80 to −0.01	nd
TH1L	Ca/Ad	1.52	4.98	(−0.77 to 1.39)/(−1.06 to −0.15)	(−1.97 to 6.27)/(−3.57 to 3.72)
	20q gain/non-20q gain	1.59	6.4	(−0.59 to 1.39)/(−1. 06 to 0.10)	(−3.57 to 6.27)/(−3.57 to 3.72)
ADRM1	Ca/Ad	1.45	1.46	(−0.62 to 0.79)/(−1.14 to 0.02)	(−0.30 to 5.58)/(−1.29 to 5.34)
	20q gain/non-20q gain	1.38	2.58	(−0.69 to 0.78)/(−1.14 to 0.36)	(−1.29 to 5.58)/(−0.30 to 5.34)
C20orf20	Ca/Ad	1.36	3.08	(−0.94 to 0.49)/(−1.31 to −0.59)	(−1.32 to 2.07)/(−2.79 to 0.14)
	20q gain/non-20q gain	1.34	3.57	(−0.89 to 0.49)/(−1.31 to −0.36)	(−1.16 to 2.06)/(−2.79 to 0.35)
TCFL5	Ca/Ad	2.2	3.54	(−2.14 to 0.83)/(−2.73 to −1.17)	(2.07 to 6.94)/(−1.28 to 4.21)
	20q gain/non-20q gain	2.02	3.54	(−2.31 to 0.83)/(−2.73 to −0.93)	(−1.28 to 6.94)/(1.99 to 4.41)

*Log₂ ratio.
nd, not determined; qRT-PCR, quantitative reverse transcription-PCR.