Table 1 PCR primers
PrimersSequences
GAPDHForward: 5′-GGTGGGTGGTCCAAGGTTTC-3′
Reverse: 5′-TGGTTTGACAATGAATACGGCTAC-3′
ZO-1Forward: 5′-CCACCGGAGTCTGCCATTACACG-3′
Reverse: 5′-GGGTGGGCTCCTCCAGTCTGACATTAG-3′
OccludinForward: 5′-GGCGGATATACAGACCCAAGAG-3′
Reverse: 5′-GATAATCATGAACCCCAGGACAAT-3′
ZO-2Forward: 5′-CCATGGGCGCGGACTATCTGA-3′
Reverse: 5′-CTGTGGCGGGGAGGTTTGACTTG-3′
ZO-3Forward: 5′-AAGCACGCAATCCTGGATGTCACC-3′
Reverse: 5′-GTCGCGCCTGCTGTTGCTGTATTA-3′
Claudin-1Forward: 5′ - AGCCAGGAGCCTCGCCCCGCAGCTGCA-3′
Reverse: 5′-CGGGTTGCCTGCAAAGT-3′

  • GADPH, glyceraldehyde phosphate dehydrogenase.