Low passage CD strictured myofibroblasts | Low passage CD non-strictured myofibroblasts | High passage CD non-strictured myofibroblasts | Low passage control myofibroblasts | High passage control myofibroblasts | |
Senescence rate (%) | 97.0 (4.1) | 25.1 (4.5)* | 95.7 (5.6) | 24.8 (5.1)* | 92 (4.9) |
TGFβ transcripts (RU) | 71.9 (8.4)† | 15.1 (2.9) | 16.7 (3.5) | 13.3 (2.7) | 12.9 (3.7) |
Collagen production (μg/ml) | 402 (79)‡ | 116 (30) | 105 (37) | 98 (29) | 101 (32) |
MMP-3 production (AU) | 5.6 (1.0) | 8.4 (1.1) | 7.4 (1.4) | 9.7 (1.3) | 8.9 (1.5) |
MMP-12 production (AU) | 0.5 (0.1) | 1.2 (0.3) | 1.1 (0.4) | 1.4 (0.6) | 1.3 (0.5) |
TIMP-1 production (AU) | 2.5 (0.5)§ | 0.9 (0.2) | 0.8 (0.3) | 0.8 (0.2) | 1.1 (0.2) |
pSmad2–3/Smad3 expression (AU) | 2.7 (0.8)¶ | 0.8 (0.1) | 0.7 (0.2) | 0.3 (0.1) | 0.6 (0.2) |
Smad7/β-actin expression (AU) | 3.2 (0.9) | 5.0 (1.3) | 4.5 (1.4) | 6.2 (1.5) | 5.2 (1.8) |
*p<0.001 vs low passage strictured cells and high passage non-strictured and control cells; †p<0.001 vs low and high passage non-strictured and control cells; ‡p<0.001 vs low and high passage non-strictured and control cells; §p<0.05 vs low and high passage non-strictured and control cells; ¶p<0.05 vs low and high passage non-strictured and control cells. Results are mean (SD).
AU, arbitrary units; CD, Crohn’s disease; MMP, matrix metalloproteinase; RU, relative units; TGFβ1, transforming growth factor β1; TIMP-1, tissue inhibitor of metalloproteinase 1.