Primary antibodies
| Antibody | Host/clone/isotype | Epitope | Concentration | Source/reference |
| αG | rabbit | α-Gliadin | 1:100 | 15 |
| Gliadin | rabbit | Gliadin | 1:50 | Sigma, St Louis, Missouri, USA |
| WB8 | m/WB8/IgG1 | GQQQPFPPQ | 1:20 | 16 |
| R5 | m/R5/IgG2a | QQQPFP | 1:25 | 24 25 |
| LAMP-2 | m/H4B4/IgG1 | Human LAMP-2 | 1:20 | Pharmingen, San Diego, California, USA |
| LAMP-1 | m/BB6 | Human LAMP-1 | 1:10 | M Fukuda, San Diego, California, USA |
| LAMP-2 | m/CD3 | Human LAMP-2 | 1:50 | M Fukuda, San Diego, California, USA |
| LAMP | rabbit | Human LAMP-1 and -2 | 1:10 | M Fukuda, San Diego, California, USA |
| Cathepsin D | rabbit | Human cathepsin D | 1:40 | Dako, Hamburg, Germany |
| HLA-DR/DP/DQ | m/CR3/43/IgG1 | β-Chain | 1:5 | J Cordell, Oxford, UK |
| HLA-DR* | m/L243/IgG2a | α- and β-Chain, HLA-DR | 1:5 | Becton & Dickinson, Heidelberg, Germany |
| HLA-DQ | m/SPV-L3/IgG2a | α-Chain, HLA-DQ | 1:5 | Serotec, Oxford, UK |
| MHC class II | rabbit | α-Chain | 1:20 | PJ Peters, Amsterdam, The Netherlands |
↵* Biotinylated.
WB8 was raised against the gliadin peptide B3144, which comprises the amino acid sequence 3–56 of A-gliadin. For immunofluorescence experiments the primary antibodies were used at up to 10 times higher dilutions as indicated.
HLA, human leucocyte antigen; IgG, immunoglobulin G; LAMP, lysosomal-associated membrane protein; m, mouse.