Table 1

Primers used for real-time quantification

Specificity	Primer name and sequences (5′–3′)	Approximate size of amplicon	Annealing temperature	Ref
β-Actin	Actin F: GCTGTGCTGTCCCTGTATGCCTCT	206	60°C	34
β-Actin	Actin R: CCTCTCAGCTGTGGTGGTGAAGC	206	60°C	34
All bacteria	Eub338F: ACTCCTACGGGAGGCAGCAG	200	60°C	35
All bacteria	Eub518R: ATTACCGCGGCTGCTGG	200	60°C	35
Bacteroidetes	Bact934F: GGARCATGTGGTTTAATTCGATGAT	126	60°C	35
Bacteroidetes	Bact1060R: AGCTGACGACAACCATGCAG	126	60°C	35
Firmicutes	Firm934F: GGAGYATGTGGTTTAATTCGAAGCA	126	60°C	35
Firmicutes	Firm1060R: AGCTGACGACAACCATGCAC	126	60°C	35
Bacteroides–Prevotella	Bac32F: AACGCTAGCTACAGGCTT	271	55°C	36
Bacteroides–Prevotella	Bac303R: CCAATGTGGGGGACCTTC	271	55°C	36
Faecalibacterium prausnitzii	Fprau223F: GATGGCCTCGCGTCCGATTAG	199	60°C	37
Faecalibacterium prausnitzii	Fprau420R: CCGAAGACCTTCTTCCTCC	199	60°C	37