Time points | Number of rats | Number for NMR analysis | Number for UPLC-MS analysis | Number for faecal bacterial analysis | Body weight (g) | t-Test p value | Food intake | t-Test p value | ||||||
RYBG (g) | ||||||||||||||
Sham | Bypass | Sham | Bypass | Sham | Bypass | Sham | Bypass | Sham | Bypass | Sham | Bypass | |||
Week -1 (Pre-op) | 18 | 18 | 18 | 18 | 6 | 6 | 0 | 0 | 341.9±30.7 | 346.6±35.8 | 0.71 | 24.7±2.8 | 25.7±2.7 | 0.31 |
2 (Post-op) | 16 | 15 | 16 | 15 | 0 | 0 | 6 | 6 | 358.6±22.6 | 288.6±29.1 | <0.001 | 29.5±2.8 | 14.3±3.2 | <0.001 |
4 (Post-op) | 16 | 15 | 16 | 15 | 0 | 0 | 383.8±21.7 | 275.6±25.4 | <0.001 | 30.9±2.7 | 22.4±3.1 | <0.001 | ||
6 (Post-op) | 16 | 14 | 16 | 14 | 6 | 6 | 0 | 0 | 400.3±22.8 | 270.9±32.7 | <0.001 | 25.7±3.2 | 18.9±5.1 | <0.001 |
8 (Post-op) | 16 | 14 | 16 | 14 | 6 | 6 | 6 | 6 | 419.8±25.3 | 259.1±36.1 | <0.001 | 20.9±4.9 | 14.6±6.5 | 0.007 |
NMR, nuclear magnetic resonance; UPLC-MS, ultra-performance liquid chromatography-tandem mass spectrometry; RYGB, Roux-en-Y gastric bypass.