E coli uptake and replication within J774-A1 murine macrophages
| Strain | Uptake of bacteria* (CFU/well×104) | Fold replication 6 h/3 h† | Fold replication 24 h/3 h† |
|---|---|---|---|
| EPI300-T1 | |||
| + pCC1FOS | 9.3±1.6 | 1.70±0.23 | 0.69±0.16 |
| + pCC1FOS/pUC18 | 11.0±2.1 | 0.86±0.05 | 1.21±0.12 |
| + pCC1FOS/pUC Afa | 0.6±0.1‡ | 1.07±0.06 | 0.27±0.10 |
| HM358 | 5.36±1.1 | 17.66±3.80§ | 11.26±2.67§ |
Data expressed as means±SEM, determined from N=2–7 independent experiments, with each experiment performed with n=2–3 replicates.
*CFU recovered from lysed macrophages after 3 h (2 h infection followed by 1 h gentamicin treatment).
†Recovered intracellular bacteria from lysed macrophages after 6 h or 24 h, relative to intracellular numbers at 3 h. Statistical analysis was performed using Mann–Whitney U with Bonferroni correction.
‡Significantly different from EPI300-T1/pCC1FOS+pUC18; p<0.0001.
§Significantly different from EPI300-T1/pCC1FOS; p<0.0001.
CFU, colony-forming units.