Table 2

Primers and probes for species-specific real-time PCR

SpeciesOligonucleotideSequence 5′-3’Targeting geneAmplicon length (bp)Reference or source
F prausnitzii
A2-165
Forward primerCCCGGCATCGGGTAGAG16S rRNA gene55Joossens et al. (2011)24
Reverse primerGGACGCGAGGCCATCTC
ProbeAAAAGGAGCAATCCGCT
B longum
LMG 13 197
Forward primerTGCCGGATGTTCCAGTTGA16S rRNA gene59This study
Reverse primerATCCCATACCGCGAAAGCT
ProbeCGCATGGTCTTCTGGGA
R hominis A2-183Forward primerGCTTCCGTGCGTGCTTGTRHOM_14 695 gene57This study
Reverse primerCCGTACTTCACGGCAAGCTT
ProbeCTTTCCGCAGTGTCCA