Table 2

Expression of GLT and IGH in jejunal mucosa biopsies and peripheral blood of H and IBS-D participants

	Jejunal mucosa			Blood
GLT	H (n=10)	IBS-D (n=12–16)	p Value	H (n=10)	IBS-D (n=9)	p Value
μGLT	1.01±0.21	1.32±0.28	0.002†^*	1.00±0.22	1.30±0.21	0.007
γ3GLT	0.99±0.35	1.74±0.76	0.002†^*	0.99±0.44	0.91±0.26	0.643
γ1GLT	0.80 (0.40–1.80)	1.90 (1.10–2.90)	0.001*	1.06±0.20	1.36±0.30	0.032
αGLT	1.00±0.71	1.72±0.97	0.202	1.00±0.62	0.80±0.22	0.624
γ2GLT	0.99±0.20	1.54±0.15	0.002*	1.00±0.57	1.33±0.34	0.223
γ4GLT	1.00±0.51	1.69±1.26	0.241	0.98 (0.60–1.40)	2.30 (0.60–8.20)	0.194
εGLT	1.01±0.50	0.98±0.46	0.904†	1.00±0.13	1.04±0.20	0.639

IGH	H (n=14)	IBS (n=16)	p Value	H (n=10)	IBS (n=9)	p Value

IGHM	1.01±0.20	1.02±0.27	0.827	1.00±0.21	0.99±0.15	0.934
IGHG3	1.01±0.38	1.37±0.46	0.023†	0.98 (0.52–1.45)	0.56 (0.49–1.9)	0.347
IGHG1	0.98±0.27	1.51±0.39	<0.001†^**	1.00±0.16	0.93±0.14	0.345
IGHA	1.00±0.15	1.09±0.23	0.205	1.00±0.29	0.85±0.15	0.195
IGHG2	1.00±0.31	1.29±0.42	0.043	0.99±0.18	0.80±0.12	0.019
IGHG4	1.00 (0.40–1.60)	1.55 (1.00–2.10)	<0.001†^**	0.99±0.27	1.04±0.13	0.595
IGHE	1.01±0.33	1.42±0.42	0.009†^**	0.99±0.33	0.74±0.19	0.065

GAPDH was used as a house-keeping gene. GLT and IGH bands were quantified by densitometric analysis and the expression of each transcript was calculated with respect to the corresponding GAPDH value. PCR products were identified by size, gel-extracted and sequenced to confirm their identity (data not shown).
p Values considered significant are shown in bold. p Value adjusted (false discovery rate): *<0.05; **<0.01.
†Differential expression between atopic and non-atopic subjects in the IBS-D group (figure 3 and see online supplementary figure 2).
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GLT, germline transcript; H, healthy subjects; IBS-D, diarrhoea-predominant IBS; IGH, Ig heavy chain.