Table 1

Gene expression of neuronal and inducible NOS

Age (days)GeneControlBB-DPp ValueBB-DPHp Value
70nNOS-α a1±0.391.85±1.35P=0.4  
 nNOS-α b1±0.312.10±1.11P=0.4  
 nNOS-α c1±0.123.74±1.85P=0.3  
220nNOS-α a1±0.120.73±0.37P=0.60.86±0.13P=0.4
 nNOS-α b1±0.160.76±0.34P=0.80.55±0.11P=0.1
 nNOS-α c1±0.250.87±0.33P=0.91.09±0.23P=0.8
  • Gene expression of nNOS splice variants (nNOSα and nNOSβ) and iNOS was measured in control, BB-DP and BB-DPH rats of 70 and 220 days (n=6) by the use of real-time reverse transcriptase PCR. Data were normalised to the housekeeping gene, hypoxanthine phosphoribosyltransferase and expressed as fold change. Data in this table represent the fold change of BB-DP and BB-DPH rats, which is compared with a fold change of one in control rats.

  • BB-DP, BioBreeding diabetes prone; BB-DPH, hyperglycaemic diabetes-prone BB rats; nNOS, neuronal isoform of nitric oxide synthase; iNOS, inducible isoform of nitric oxide synthase.