Pulmonary cytokine concentrations in untreated and microbiota-depleted mice during pneumococcal pneumonia
| Time after Streptococcus pneumoniae infection | |||
|---|---|---|---|
| 6 h | 24 h | 48 h | |
| TNF-α | |||
| Untreated | 2038 (1306–2130) | 1950 (1722–2449) | 123.6 (109.5–174.9) |
| Microbiota depleted | 1021 (707.3–1091)** | 1074 (929.4–1277)** | 152.3 (134.5–494.5) |
| IL-1 | |||
| Untreated | 96.6 (52.6–106.5) | 62.4 (57.9–236.1) | 48.7 (43.5–53.7) |
| Microbiota depleted | 120.9 (89.7–128.2)* | 109.3 (80.0–204.8) | 64.5 (47.7–122.0) |
| IL-6 | |||
| Untreated | 71.4 (42.0–86.5) | 80.4 (42.1–538.4) | 105.2 (98.4–125.5) |
| Microbiota depleted | 110.8 (90.2–152.9)* | 100.7 (52.2–192.9) | 117.1 (99.6–312.8) |
| IL-10 | |||
| Untreated | 41.3 (29.9–60.84) | 75.7 (45.0–91.4) | 48.4 (30.7–77.3) |
| Microbiota depleted | 21.4 (10.5–27.4)* | 45.2 (19.6–52.9) | 51.43 (43.7–80.4) |
| CXCL1 | |||
| Untreated | 125.5 (68.5–147.4) | 87.8 (60.4–839.1) | 54.3 (44.5–226.8) |
| Microbiota depleted | 247.3 (155.1–455.1)** | 132.1 (59.1–448.1) | 44.3 (23.9–134.8) |
Data are expressed as median (IQR) of n=8 mice per group per time point (6 h, 24 h or 48 h post intranasal challenge with S. pneumoniae). Measurements are expressed in pg/mL.
**p<0.01 for Untreated versus Microbiota-depleted mice (Mann–Whitney U test).
*p<0.05 for Untreated versus Microbiota-depleted mice (Mann–Whitney U test).
CXCL1, (C-X-C motif) ligand 1; IL, interleukin; TNF-α, a tumour necrosis factor-α.