Oligonucleotides used for PCR experiments
| Primer | Oligonucleotide sequence (5′–3′) | PCR product size (bp) | Use |
| A2GBL-3 | AAAGCCACGTTGTGTCTCAA | 957 | Kanamycin resistance cassette amplification |
| B2GBLnp5 | TTAGAAAAACTCATCGAGCA | ||
| MIompA(R) | AAAGGCAAAAAAAACCCCGCAGCGGGGTTTTTCTACCAGACGAGAACTTAGAAAAACTCATCGAGCA | 1097 | ΔompA isogenic mutant construction |
| MIompA(F) | CTCGTTGGAGATATTCATGGCGTATTTTGGATGATAACGAGGCGCAAAAAAAAGCCACGTTGTGTCTCAA | ||
| OmpA1 | GGAGCCGGAGCAACTACTGG | 205 | Isogenic mutant verification |
| OmpA2 | ACGACACCGGCGTTTCTCCG | ||
| OmpA3 | GCAGGCATTGCTGGGTAAGG | 1232 | Isogenic mutant verification and sequencing |
| OmpA4 | AATATTGAGCAGATCCCCCGG | ||
| OmpASalI | ACGCGTCGACCGTTGGAGATATTCATGGCG | 1098 | Cloning of ompA gene |
| OmpAHindIII | CCCAAGCTTGGGAGACGAGAACTTAAGCCTGC | ||
| gp96-upstream | GGGTGTGGTGGACTCAGATG | 669 | gp96 quantification by qRT–PCR48 |
| gp96-downstream | GTTGCCAGACCATCCGTACT | ||