Study | Subject | Sample | Method | Patient group | Main finding | Country of study |
Balsari et al96 |
| Faeces | Culture | IBS |
| Italy |
Si et al98 |
| Faeces | Culture | IBS |
| China |
Malinen et al99 |
| Faeces | qPCR | IBS |
| Finland |
IBS-D |
| |||||
IBS-C |
| |||||
Mättö et al100 |
| Faeces | CulturePCR-DGGE | IBS |
| Finland |
Maukonen et al101 |
| Faeces |
| IBS |
| Finland |
IBS-C |
| |||||
Kassinen et al102 |
| Faeces |
| IBS |
| Finland |
Rajilić-Stojanović103 |
| Faeces | Microarray | IBS |
| Finland |
Kerckhoffs et al104 |
|
|
| IBS |
| The Netherlands |
Krogius-Kurikka et al105 |
| Faeces | GC-profiling + sequencing of 16S rRNA genes | IBS-D |
| Finland |
Lyra et al106 |
| Faeces | qPCR | IBS-D |
| Finland |
IBS-C |
| |||||
IBS-A |
| |||||
Tana et al107 |
| Faeces |
| IBS |
| Japan |
Codling et al108 |
|
| PCR-DGGE | IBS |
| Ireland |
Carroll et al28 |
|
|
| IBS-D |
| USA |
Noor et al109 |
| Faeces | PCR-DGGE + sequencing of 16S rRNA genes | IBS |
| UK |
Malinen et al110 |
| Faeces | qPCR |
| Finland | |
Ponnusamy et al111 |
| Faeces | DGGE + qPCR of 16sRNA genes |
| Korea | |
Rinttila et al112 |
| Faeces | qPCR | IBS |
| Finland |
Saulnier et al113 |
| Faeces | 16s Metagenomic sequencing and DNA microarray | IBS |
| USA |
Rajilic-Stojanovic et al114 |
| Faeces | Phylogenetic 16S rRNA microarray and qPCR | IBS |
| Finland |
Carroll et al115 |
| FaecesColonic mucosa | T-RFLP fingerprinting of 16S rRNA - PCR | IBS-D | Diminished microbial biodiversity in faecal samples | USA |
Parkes et al116 |
| Colonic mucosa |
| IBS | Expansion of mucosa-associated microbiota; mainly bacteroides and clostridia; association with IBS subgroups and symptoms | UK |
Jeffery et al117 |
| Faeces | Pyrosequencing 16SrRNA | Clustering of IBS patients—normal-like versus abnormal microbiota composition (increased ratio of Firmicutes to Bacteroidetes); association with symptom profile | Sweden |
n, number of randomised subjects.
B, Bifidobacterium; C, constipation; C, Clostridium; Cl, Clostridium; ctrls, controls; D, diarrhoea; DGGE, denaturing gradient gel electrophoresis; FISH, fluorescent in situ hybridisation; IBS, irritable bowel syndrome; L, Lactobacillus; qPCR, quantitative PCR; R, Ruminococcus; S, Staphylococcus; T-RFLP, terminal restriction fragment length polymorphism.