Table 1

Mutated CAN-genes found in eight massively sequenced CRC cell lines

Tumour cell lines
CAN genes10391076
Diff CSC		1247
Diff CSC		15461869187221 05223 873
APCS1395fs*2V915fs*2V915fs*2R1114*d (44%)e
R1450* (52%)
R2204* (33%)
A2650V (58%)		R1114* (44%)
R1450* (52%)
R2204* (33%)
A2650V (58%)		Q1328*E1317fs*3E893*
KRASG12DG12DG12DQ61LG13DG12DG12CG13D
TP53Y107DR248WR248WR175HR175HR248QR158L
PIK3CAQ546KR770QR770QE545K
FBXW7R479QH379Y
CSMD3L2424fs*9
TNN
NAV3
SMAD4V370A/E41*V370A/E41*R361CS325P
EPHA3
MAP2K7
EPHRB6173insSSC316YC316Y
PTEN
ADAMTSL3
GUCY1A2
SMAD2G457*
OR51E1
LAMA1
C10orf137S168LS168L
TCF7L2P477TP477T

  • *d, nonsense mutation introducing stop codon; e (percentage of variation), indicated the frequency of cDNA reads containing the mutation encoding the reported protein sequence, determined on all the APC cDNA sequences obtained.

  • APC, antigen presenting cells; CSC, cancer stem/initiating cell cultures; CRC, colorectal cancers; Diff, differentiated cancer cell lines; fs*, frameshift mutation followed by nucleotide number before stop codon.