Table 2

The mutation D476G occurred during H06 IgG treatment in vivo and conferred antibody resistance against H06 IgG and three monoclonal antibodies, AR3–5, in vitro.

AntibodyIC50 value (µg/mL)
J6/JFH1J6/JFH1D476GFold change
H06 IgG110073006.6
  • Dose–response neutralisation was performed as described using HCV with J6 envelope proteins with and without D476G. Dose–response curves were analysed using GraphPad PRISM (V.4.03) as described in ‘Materials and Methods’.