Table 2

The mutation D476G occurred during H06 IgG treatment in vivo and conferred antibody resistance against H06 IgG and three monoclonal antibodies, AR3–5, in vitro.

Antibody	IC₅₀ value (µg/mL)
Antibody	J6/JFH1	J6/JFH1_D476G	Fold change
H06 IgG	1100	7300	6.6
AR3A	19	48	2.5
AR4A	7.4	43	5.8
AR5A	43	88	2.0

Dose–response neutralisation was performed as described using HCV with J6 envelope proteins with and without D476G. Dose–response curves were analysed using GraphPad PRISM (V.4.03) as described in ‘Materials and Methods’.