Table 1.

Somatic mutations identified in pancreatic juice by digital NGS*

					KRAS		GNAS		RNF43		TP53		SMAD4
Case#	M/F	Age	Disease group	Risk**	dNGS#	SNV	dNGS#	SNV	dNGS#	SNV	dNGS#	SNV	dNGS#	SNV
#01	F	27	Control	na	0		0		0		0		0
#02	F	79	Control	na	1	p.G12D	0		0		0		0
#03	F	77	Control	na	0		0		0		0		0
#04	M	50	Control	na	1	p.G12D	0		0		0		0
#05	F	78	Control	na	0		0		0		0		0
#06	M	76	Control	na	0		0		0		0		0
#07	F	49	Control	na	1,2,2	p.G12D/V,G13D	0		0		0		0
#08	F	65	Control	na	0		3	p.R201C	3	p.Q402R	0		0
#09	M	45	Control	na	1,2	p.G12D/V	0		0		0		0
#10	F	68	Control	na	0		0		0		0		0
#11	F	48	Control	na	0		0		0		0		0
#12	F	43	Control	na	0		0		0		0		0
#13	F	44	Control	na	0		0		0		0		0
#54	M	46	Control	na	0		0		0		0		0
#55	F	72	Control	na	2	p.Q61L	0		0		0		0
#56	M	34	Control	na	1	p.Q61L	0		0		0		0
#57	F	52	Control	na	1	p.G12S	1	p.R201H	0		0		0
#58	M	66	Control	na	0		2,2	p.R201C/H	0		0		0
#59	F	59	Control	na	1	p.G12S	0		0		0		0
#60	M	59	Control	na	1,1	p.G12D/S	0		0		0		0
#61	F	73	Control	na	0		0		0		0		0
#62	M	48	Control	na	0		0		0		0		0
#63	M	40	Control	na	0		1	p.R201C	0		0		0
#64	F	32	Control	na	1	p.G12D	0		0		0		0
#15	M	64	IPMNs	f	1	p.G12V	0		0		0		0
#23	F	64	IPMNs	s	3,4	p.G12D/V	0		0		0		0
#24	M	21	IPMNs	s	3	p.G13D	29	p.R201C	10,3	p.R343H, Y550C	0		0
#25	M	67	IPMNs	s	0		0		0		0		0
#26	M	51	IPMNs	f	0		4,1	p.R201C/H	4,3	p.P634S, Q402X	3	p.R196L	0
#27	M	62	IPMNs	f	0		0		0		2	p.R248Q	0
#28	M	70	IPMNs	s	1	p.G12D	4,1	p.R201C/H	0		4	p.K291R	0
#29	F	75	IPMNs	s	1	p.G12V	2	p.Q227R	0		0		0
#30	F	63	IPMNs	s	1,2,4	p.G12D/V/R	0		3	p.S446F	0		0
#65	F	58	IPMN	s	1,1,1	p.G12D/V,Q61L	1	p.Q227R	0		0		0
#66	M	67	IPMN	s	9	p.G12C	0		0		0		0
#67	M	70	IPMN	s	1,1	p.G12D/V	2	p.R201C	0		0		0
#68	M	59	IPMN	s	1	p.G12V	0		3	p.S464G	0		0
#69	F	65	IPMN	s	1,1	p.G12S, Q61R	1	p.R201C	3	p.S478P	0		0
#70	M	62	IPMN	s	1	p.Q61R	0		0		3	p.M246V	0
#71	F	84	IPMN	s	0		0		0		0		0
#72	M	66	IPMN	s	1,2	p.G12V, Q61H	1	p.R201H	0		0		0
#73	F	60	IPMN	s	1	p.Q61L	0		0		0		0
#74	F	66	IPMN	s	1,1,1	p.G13D, Q61H/R	0		0		0		0
#75	M	70	IPMN	s	0		0		0		0		0
#76	M	41	IPMN	s	1,1	p.G12V, Q61R	0		0		2	p.R175H	0
#77	F	55	IPMN	s	1	p.Q61R	0		0		0		0
#78	F	74	IPMN	s	1,1,1	p.G12C, G13D, Q61R	0		3	p.A148V	3	p.R175H	0
#79	M	76	IPMN	s	0		1	p.Q227R	5	p.A629T	0		0
#80	F	64	IPMN	s	1,8	p.G12V, Q61H	8	p.R201C	0		0		0
#81	F	44	IPMN	s	2,1,1,1,1	p.G12D/V/R, p.Q61H/R	1	p.R201H	0		0		0
#82	F	77	IPMN	s	1	p.Q61H	1	p.R201H	0		0		0
#83	F	67	IPMN	s	2,1,1,2,1	p.G12D/V/R/S, G13D	0		0		0		0
#84	M	84	IPMN	s	1,1	p.G12C/S	1	p.R201H	3	p.G263E	0		0
#85	M	49	IPMN	s	1,2	p.G12D/V	0		0		0		0
#86	F	74	IPMN	s	1,1,1	p.G12D, Q61H/R	1	p.R201C	3	p.L122P	3	p.Q165X	0
#87	F	77	IPMN	s	1,2,1,1	p.G12D/V/S, Q61L	0		0		0		0
#88	F	83	IPMN	s	2	p.G12V	1	p.R201H	0		2	p.R282W	0
#89	F	83	IPMN	s	0		1	p.R201H	0		4	p.R248W	0
#90	M	70	IPMN	s	1,1	p.G12D, Q61H	1	p.Q227P	0		2	p.G245S	0
#91	F	62	IPMN	s	1,1,2	p.G12V/R/S	3	p.R201C	0		0		0
#92	F	70	IPMN	s	5,4,2	p.G12D/V/C	3,1	p.R201C, Q227R	0		0		0
#93	M	62	IPMN	s	1,1,1	p.G12C/S, Q61H	0		0		0		0
#94	F	66	IPMN	s	0		0		0		0		0
#95	M	64	IPMN	s	0		2	p.R201C	0		0		0
#96	M	76	IPMN	s	0		0		0		2	p.R248W	0
#97	F	83	IPMN	s	13	p.G12V	1,1	p.R201C/H	0		0		0
#98	F	68	IPMN	s	0		0		0		2	p.Q136X	0
#99	F	60	IPMN	s	1,1	p.Q61L/R	1	p.R201H	3	p.S111G	0		0
#14	M	48	LG-IPMN	s	0		1	p.R201H	4	p.A618V	0		0
#16	F	63	LG-IPMN	f	2	p.G12D	0		0		0		0
#17	F	60	LG-IPMN	f	5,8,1,3,2	p.G13D, G12C/R/V, Q61R	8	p.R201H	15	p.L12Rfs	2	p.R248Q	0
#18	M	75	LG-IPMN	f	1	p.G12V	2,1	p.Q227R, R201C	0		0		0
#100	M	78	LG-IPMN	f	1,2	p.G13D, Q61R	1	p.Q227R	0		2	p.R175H	0
#101	M	72	LG-IPMN	f	0		0		0		0		0
#19	F	67	IM-IPMN	s	2	p.G13D	2	p.R201C	0		0		0
#20	F	73	IM-IPMN	s	0		2	p.R201C	0		2	p.R282W	0
#21	F	67	IM-IPMN	s	1	p.G12V	1	p.R201C	0		0		3	p.W524R^
#22	M	65	IM-IPMN	f	2	p.G12V	0		0		0		0
#102	M	72	IM-IPMN	s	5	p.Q61H	0		3	p.R127Q	0		0
#103	F	75	IM-IPMN	f	7,1,18,1,18	p.G12A/V/R, G13D, Q61H	1	p.R201C	0		0		0
#104	M	71	HG-IPMN	f	1,1,2,1,7,4,1	p.G12D/R/S, G13D, p.Q61L/H/P	0		0		0		0
#31	M	79	PDAC/IPMNs	s	1	p.G12V	0		0		2	p.R196X	0
#32	M	69	PDAC/IPMNs	s	1	p.Q61R	0		0		3	p.R175H	0
#33	F	74	PDAC/IPMNs	s	4,2	p.G13D, G12R	1	p.R201H	0		0		0
#34	F	55	PDAC/IPMNs	s	23,6,3,2,2,2,2,2	p.G12D/S/R/C/V, G13C/D	0		3	p.R454H	26	p.R248Q	0
#35	M	65	PDAC/IPMNs	f	1	p.G12V	0		0		3	p.R248Q	0
#36	M	74	PDAC/IPMNs	f	21	p.G12D	2	p.R201C	0		0		26,4	p.Q311X, Q256X
#37	M	69	PDAC/IPMN	s	0		61	p.R201C	0		87	p.L194R	0
#38	M	73	PDAC/IPMNs	s	12	p.G12D	1,1	p.R201C/H	0		0		0
#105	M	68	PDAC/IPMNs	s	1	p.G12D	6	p.R201C	3,3	p.F322S, H295R	8	p.C141G	0
#106	M	44	PDAC/IPMNs	s	10	p.Q61R	0		0		12	p.R175H	3	p.T273fs
#107	M	59	PDAC/IPMN	s	8,8	p.G12D/S	0		10	p.P470fs	2	p.R175H	0
#115	M	68	PDAC/IPMN	s	6,1,1	p.G12C/S, Q61L	1	p.R201H	0		3	p.R181C	0
#39	M	70	PDAC	f	0		0		0		0		0
#40	M	53	PDAC	s	0		0		0		6	p. L130Sfs	0
#41	F	46	PDAC	s	3,6	p.G12D/R	2	p.R201H	4	p.C471R	0		0
#42	M	78	PDAC	s	6,2	p.G13D, G12V	1	p.R201C	0		0		0
#43	F	59	PDAC	s	3,5	p.G12S/V	0		0		2	p.R248W	0
#44	F	77	PDAC	s	1	p.G12D	2	p.R201C	5	p.H556R	3	p.R175H	7	p.A457V^
#45	M	56	PDAC	s	6,2	p.G12D/V	0		4	p.R519X	7	p.Y220C	0
#46	M	58	PDAC	s	17	p.G12D	0		0		0		0
#47	F	75	PDAC	s	32,22,4,4	p.G13D, Q61H, G12D/R	0		0		2	p.R273C	0
#48	M	59	PDAC	f	0		0		3,3	p.L109P, V480A	3	p.C277R	3	p.M543T^
#49	F	69	PDAC	s	23,2	p.G12V/S	2	p.R201C	0		39	p.R196X	0
#50	F	68	PDAC	s	0		0		0		0		0
#51	F	66	PDAC	s	0		0		0		0		11	p.K50Kfs
#52	F	62	PDAC	f	4	p.G12D	0		0		0		0
#53	F	79	PDAC	f	1,1	p.G12D/R	0		0		4,1,1	p.Y220C, R248W/Q	0
#108	M	63	PDAC	s	0		1	p.Q227R	0		0		0
#109	M	71	PDAC	s	1	p.Q61H	0		0		0		0
#110	M	70	PDAC	f	0		0		3	p.H472R	2	p.R248W	0
#111	M	62	PDAC	s	2,1	p.G12D, G13D	0		0		0		0
#112	F	71	PDAC	s	1	p.Q61H	1	p.R201C	0		2,2	p.R248W, Y163C	0
#113	F	77	PDAC	s	11	p.G12V	1	p.R201H	0		2	p.Y220C	0
#114	M	57	PDAC	s	0		0		0		0		0

Case #20 had a BRAF K601K mutation (score 4), Case #26 had a TGFBR2 mutation D317Y (score 4), Case #53 had PIK3CA mutations H1047L/R (score 3, 6), Case#105 had a BRAF F595L mutation (score 4), Case # had a Case #107 had a PIK3CA H1047R mutation (score 3).
*, Other four genes shown in supplementary table;**, s: sporadic; f: familial. dNGS#:digital NGS score (mutation concentration); SNV:single nucleotide variant (i.e. somatic mutation). ^suspected pathogenic.