Demographic and clinical details of samples
| Cohort | Factor | Control | CRC | Adenoma | P values | Sample collection | Sequencing platform |
| Chinese cohort 1 (discovery) | Sample size | 92 | 73 | NA | NA | No antibiotics and no invasive medical intervention for 3 months; no vegetarian diet; no history of cancer or inflammatory disease of intestine | Sequencing platform: Illumina HiSeq 2000/2500; sequencing target depth: 5 GB; read length: 100 bp |
| Age | 58.51 (7.55) | 65.90 (10.61) | NA | <0.0001 | |||
| Gender | Male: 51Female: 41 | Male: 47Female: 26 | NA | 0.316 | |||
| BMI | 23.87 (3.31) | 24.07 (3.18) | NA | 0.697 | |||
| Chinese cohort 2 validation (V1) | Sample size | 112 | 111 | 197 | NA | ||
| Age | 62.39 (6.51) | 73.3 (10.50) | 66.11 (5.94) | <0.0001 | |||
| Gender | Male: 44 Female: 68 | Male: 59 Female: 52 | Male: 119 Female: 78 | 0.002 | |||
| BMI | 23.91 (3.34) | 24.29 (3.17) | 24.51 (3.48) | 0.322 | |||
| German and French validation (V2) | Sample size | 66 | 90 | 42 | NA | No previous colon or rectal surgery, CRC, inflammatory or infectious injuries of the intestine; no need for emergency colonoscopy | |
| Age | 58.75 (12.96) | 68.44 (12.22) | 62.95 (9.11) | 0.012 | |||
| Gender | Male: 32Female: 32 | Male: 53 Female: 35 | Male: 30 Female: 12 | 0.087 | |||
| BMI | 24.72 (3.19) | 25.89 (4.29) | 25.90 (4.10) | 0.056 |
BMI, Body Mass Index; CRC, colorectal cancer; NA, not applicable.