Table 2

Univariate and multivariate analysis of clinical and molecular prognostic factors in terms of RFS

Patients (%)UnivariateMultivariate
HR (95% CI)P valuesHR (95% CI)P values
Liver cirrhosis (present vs absent)106 (56)1.64 (1.00 to 2.69)0.049NS
Microscopic vascular invasion (yes vs no)81 (44)1.87 (1.16 to 3.00)0.0102.09 (1.14 to 3.83) 0.017
Aetiology (hepatitis C vs others)61 (32)1.70 (1.06 to 2.73)0.028NS
Hepatocyte pERK (positive vs negative)20 (11)2.46 (1.36 to 4.44)0.0032.41 (1.21 to 4.80) 0.012
pVEGFR2 (positive vs negative)54 (36)1.84 (1.09 to 3.09)0.022NS
G3 (present vs absent)43 (31)1.73 (1.00 to 3.00)0.049NS
MET (present vs absent)18 (13)2.28 (1.16 to 4.47)0.017NS
Response to IFNα1 (present vs absent)24 (17)2.09 (1.13 to 3.87)0.019NS
  • The univariate analysis was conducted for 33 variables, including clinicopathological variables (liver cirrhosis, microscopic vascular invasion, aetiology, multinodularity, maximum tumour size (threshold 50 mm), tumour satellites, histological grade (3 vs 1–2)), molecular traits (hepatocyte pERK, endothelial pERK, nuclear pVEGFR2 and VEGFA) and 22 gene signatures (online supplementary table 10). In the multivariate analysis, only microscopic vascular invasion and hepatocyte pERK retained independent prognostic value.

  • IFN, interferon; NS, non-significant; RFS, recurrence-free survival; VEGFA, vascular endothelial growth factor A.