Type-AB DNA methylation at gene promoters targets specific signalling pathways
| Pathway name | Fold enrichment | FDR-corrected p value |
| Axon guidance mediated by Slit/Robo | 6.81 | 1.31×10–2 |
| Axon guidance mediated by netrin | 5.45 | 1.29×10–2 |
| Ionotropic glutamate receptor pathway | 5.34 | 1.70×10–3 |
| Cadherin signalling pathway | 4.41 | 4.32×10–7 |
| 5HT1 type receptor-mediated signalling pathway | 4.34 | 3.73×10–2 |
| Metabotropic glutamate receptor group III pathway | 4.01 | 1.15×10–2 |
| Heterotrimeric G-protein signalling pathway-Gi alpha and Gs alpha mediated | 3.22 | 8.97×10–4 |
| Wnt signalling pathway | 2.65 | 2.29×10–4 |
Type-AB CpGs were identified using a two-step process: First, we identified CpGs that were significantly associated with age in both the intestine of wild-type animals and Braf mutant animals from weaning. From this subset of type-A CpGs, we identified those that (de)accumulate DNA methylation at significantly different rates according to mutation status by analysis of covariance. CpGs were assessed by regression analyses and pathway enrichment determined using the PANTHER enrichment tool.
FDR, false discovery rate.