Table 2

Location of HBV DNA integrations in coding regions of the human genome by whole exome sequencing

Patient no.Patient groupChimeric HBV human reads (n)Human chromosome no.Human genesGene description*Location of HBV integrants within human genesDistance to proximal exons†Potential downstream effect‡
HBV DNA integration in intronic or exonic regions of human genome
 1411Chr_21COL18A1§Collagene XVIIIIntronic14 091Regulation of angiogenesis and tumour growth
 2211Chr_16LMF1§Lipoprotein maturation factor 1Intronic7157Protein involved in lipoprotein metabolism
 3321Chr_19ADGRL1§G-protein coupled receptorsIntronic7310Involved in cell adhesion and signal transduction
 3931Chr_17ELAC2Ribonuclease ZIntronic15¶Altered levels in liver cancer
 4032Chr_11IFITM1Interferon-induced antiviral proteinIntronic44 and 72**B cell receptor signalling pathway
 4421Chr_17NUP85Nuclear pore complexIntronic6**RNA transport pathway; altered levels are associated with negative prognosis in liver cancer
 6238Chr_12ANKRD52§Ankyrin repeat domainIntronic6 and 32**Altered levels are associated with negative prognosis in liver cancer
 7521Chr_2AGBL5§MetallocarboxypeptidaseIntronic80¶Altered levels are associated with negative prognosis in liver cancer
 8322Chr_5NR3C1§Glucocorticoid receptorExonic0Regulation of inflammatory responses
 8432Chr_4CYP2U1Cytochrome P450Intronic99¶Drug metabolism
HBV DNA integration in intergenic regions of human genome
 232Chr_9n.a.n.a.Intergenic458 413n.a.
 1031Chr_5n.a.n.a.Intergenic69 228n.a.
 6831Chr_18n.a.n.a.Intergenic158 827n.a.
 8132Chr_10n.a.n.a.Intergenic17 498n.a.
  • The table displays the 12 HBV DNA integration events observed in the 10 patients localised in intronic or exonic regions of human genome and the 4 HBV DNA integration events localised in intergenic regions.

  • *Genes information is retrieved by GeneCards: The Human Gene Database.

  • †Distance in nucleotides of HBV integrants to the most proximal exons.

  • ‡Genes’ cellular role is retrieved from the Human Protein Database and from KEGG PATHWAY Database.

  • §These genes were involved in HBV DNA integration events as reported in Virus Integration Sites Database (https://bioinfo.uth.edu/VISDB/index.php/homepage).

  • ¶HBV integrants reside in proximity (<100 nucleotides) to donor site, localised at 5’ end of introns and crucial for RNA splicing.

  • **HBV integrants reside within or in proximity (<50 nucleotides) to branching site, a crucial region localised at 3’ end of introns and crucial for RNA splicing.

  • n.a., not applicable.