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Nested-Polymerase Chain Reaction for the Detection ofHelicobacter pyloriInfection with Novel Primers Designed by Sequence Analysis of Urease A Gene in Clinically Isolated Bacterial Strains

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Abstract

We have established a highly sensitive semi-nested PCR assay for the detection ofH. pyloriinfection using gastric juice samples, which can be aspirated with disposable nasogastric tubes. The primers targetingH. pyloriurease A gene were designed based on the sequence conservation analysis of sixteenH. pyloristrains isolated from Japanese patients. The efficacy of the PCR assay, designated as the URA-PCR, was confirmed byin vitroandin vivoassessments. Its sensitivity was 97.5% in the gastric juice samples aspirated from forty patients with provenH. pyloriinfection, and was significantly higher than that obtained with previously described PCR assays.

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Corresponding Author: Haruhiko YOSHIDA, M.D., 2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113, Japan. FAX: +81-3-3814-0021.

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