Regular ArticleMutational Events in Consecutive Passages of Hepatitis A Virus Strain GBM during Cell Culture Adaptation
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Analysis of adaptive mutations selected during the consecutive passages of hepatitis E virus produced from an infectious cDNA clone
2016, Virus ResearchCitation Excerpt :In agreement with this finding, HAV variants of p16 HM175 (16th in vitro passage level) exhibited 19 mutations (0.3% or 19/7478) in comparison with the wild-type genome (Jansen et al., 1988). Likewise, GBM/HFS displayed 24 mutations (0.3%) after 31 passages (Graff et al., 1994). However, only six nucleotide substitutions (0.14% or 6/7226) were found in the P10 genome during ten consecutive passages (348 days culture) in the present study, similar to H2K7_P12 which displayed 7 mutations (0.1%) during 12 passages (Hu et al., 2002).
Construction and characterization of 3A-epitope-tagged foot-and-mouth disease virus
2015, Infection, Genetics and EvolutionCitation Excerpt :The N-terminal half (positions 1–75) of the FMDV 3A protein, which contains an N-terminal hydrophilic domain and a hydrophobic domain (Forss et al., 1984), is highly conserved in contrast to the C-terminal half where many mutations and deletions occur among all FMDVs (Knowles et al., 2001). Changes in 3A protein have been associated with altered host range in the rhinoviruses (Heinz and Vance, 1996), enteroviruses (Lama et al., 1998), and hepatoviruses (Graff et al., 1994a, 1994b; Morace et al., 1993). In FMDV, deletions in the C-terminal half of 3A have been associated with attenuated virulence in cattle.
A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle
2013, VirologyCitation Excerpt :FMDV 3A is a conserved protein of 153 amino acids (aa) in most FMDVs examined to date. Changes in 3A have been associated with altered host range as reviewed by Knowles et al. (2001) in the hepatoviruses (Graff et al., 1994a, 1994b; Lemon et al., 1991; Morace et al., 1993), rhinoviruses (Heinz and Vance, 1996) and enteroviruses (Lama et al., 1998). The 3A protein in FMDV is substantially larger than the 3A proteins of other picornaviruses being 50% larger than the 87 aa 3A of poliovirus (Kitamura et al., 1981).
Hepatitis E virus cell culture models
2011, Virus ResearchCitation Excerpt :They all had the same genomic length of 7226 nucleotides (nt) as the wild-type JE03-1760F [the poly(A) tract at the 3′-terminus excluded]. Few genetic changes (0.3%) were found in HEV progenies thriving in cell culture similar to the adaptation of hepatitis A virus (HAV), another enterically transmitted hepatitis virus (Graff et al., 1994; Hu et al., 2002; Jansen et al., 1988). Briefly, the p5/A isolate possessed 9 mutations which were restricted to 5′UTR, ORF1 or ORF2.
Genetic characterization of Indian type O FMD virus 3A region in context with host cell preference
2010, Infection, Genetics and Evolution