Abstract
Thirty-six isolates of H. pylori from up to three gastric biopsy sites (antrum, corpus and fundus) from 13 patients in Italy with different degrees of histological gastritis were investigated. All strains were tested for motility, cytotoxicity and degree of adhesion, and were typed by analysis of ribosomal RNA gene patterns (ribopatterns). Seventeen different DNA types (ribotypes) were identified, with each patient possessing H. pylori of one or more unique types. Only two patients had identical H. pylori at three sites. Most patients had H. pylori with different ribotypes or subtypes, but nine strains were not typable. Five patients had the same strain colonizing two of the three sites and atypical strains were mostly from the antrum. A complex pattern of H. pylori colonization in the stomach of some individuals was evident and suggested multiple sources of infection. No consistent associations were detected between degree of gastritis and adherence, cytotoxicity and motility but a 2.56Kb rRNA gene fragment that had a higher frequency in strains associated with severe gastritis than mild gastritis, may provide a useful molecular marker for future pathogenicity studies.
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References
BejiA., VincentP., DachisI., HussonM.O., CortolA. and LeclercH. (1989): Evidence of gastritis with several Helicobacter pylori strains - Lancet ii: 14021–403.
CowanS.T. (1974): Cowan and Steeles' Manual for the identification of medical bacteria, 2nd ed. -Cambridge University Press, London.
DyeK., MarshallB.J., Friesson, H.F., PambiancoD.J. and McCallumR.W. (1990): Campylobacter pylori colonizing heterotopic gastric tissue in the rectum -Am. J. Clin. Pathol. 93: 144–147.
FiguraN., GuglielmettiP., RossoliniA.et al. (1989): Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only - J. Clin. Microbiol. 27: 225–226.
FormanD., NewellD.G., FullertonF.et al. (1991): Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation - Br. Med. J. 302: 1302–1305.
GrahamD.Y. (1989): Campylobacter pylori and peptic ulcer disease - Gastroenterology 96: 615–625.
KrajdenS., FuksaM., AndersonJ., KempstonJ., BocciaA., PetreaC.et al. (1989): Examination of human stomach biopsies, saliva, and dental plaque for Helicobacter pylori - J. Clin. Microbiol. 27: 1397–1398.
LangenbergW., RauwsE.A.J., WidjojokusumoA., TytgatG.N.J. and ZanenH.C. (1986): Identification of Helicobacter pylori isolates by restriction endonuclease DNA analysis - J. Clin Microbiol. 24: 414–417.
MajewskiS.I.H. and Goodwin C.S. (1988): Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation - J. Infect Dis. 157: 465–471.
MajmudarP., ShahS.M., DhunjebhoyK.R. and DesaiH.G. (1990): Isolation of Helicobacter pylori from dental plaques in healthy volunteers - Indian J. Gastroenterol. 9: 271–272.
MorganD.D. and OwenR.J. (1990): Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from humans and animal hosts - Mol. Cell. Probes 4: 321–334.
OudbierJ.H., LangenbergW., RauwsE.A.J. and Bruin-MoschC. (1990): Genotypical variation of Campylobacter pylori from gastric mucosa - J. Clin. Microbiol. 28: 559–565.
OwenR.J., OnS.L.W. and CostasM. (1988): Potential transport medium for Campylobacter pylori - J. Clin. Pathol. 41: 1337–1339.
OwenR.J., FraserJ., CostasM., MorganD. and MorganD.R. (1990): Signature patterns of DNA restriction fragments of Helicobacter pylori before and after treatment - J. Clin. Pathol. 43: 646–649.
OwenR.J., BickleyJ., CostasM. and MorganD.R. (1991): Genomic variation of Helicobacter pylori: application to identification of strains - Scand. J. Gastroenterol. 26 (suppl. 181): 43–50.
OwenR.J., BickleyJ., LastovicaA., DunnJ.P., BormanP. and HuntonC. (1992): Ribosomal RNA gene patterns of Helicobacter pylori from surgical patients with healed and recurrent peptic ulcers - Epidemiol. Infect. 108: 39–50.
OwenR.J., FiguraN. and MorenoM. (1992): Biotypes and DNA fingerprints of cytotoxigenic Helicobacter pylori from patients with gastritis and peptic ulcerations in Italy - Eur. J. Epidemiol. 8: 15–21.
OwenR.J., HuntonC., BickleyJ., MorenoM. and LintonJ.D. (1992): Ribosomal RNA gene restriction patterns of Helicobacter pylori: analysis and appraisal of HaeIII digests as a molecular typing system -Epidemiol. Infect. 109: 35–47.
OwenR.J. (1993): Microbiology and epidemiology of Helicobacter pylori infections - CDR Rev. 3: R51-R56.
OwenR.J., BellG.D., DesaiM., MorenoM., GantP.W., JonesP.H. and LintonD. (1993): Biotype and molecular fingerprints of metronidazole resistant strains of Helicobacter pylori from antral gastric mucosa - J. Med. Microbiol. 38: 6–12.
ParsonnetJ. (1989): The epidemiology of C. pylori. In: Campylobacter pylori gastritis and peptic ulcer disease. Blaser M.J. (Ed.) - Igaku Shoin Medical Publishers, New York. pp. 51–60.
PetersonW.L. (1991): Helicobacter pylori and peptic ulcer disease - New Engl. J. Med. 324: 1043–1048.
PitcherD.G., OwenR.J., DyalP. and BeckA. (1987): Synthesis of a biotinylated DNA probe to detect ribosomal RNA cistrons in Providencia stuartii -FEMS Microbiol. Lett. 48: 283–287.
PitcherD.G., SaundersN.A. and OwenR.J. (1989): Rapid extraction of bacterial genomic DNA with guanidium thiocyanate - Lett. Appl. Microbiol. 8: 151–156.
PrewettE.J., BickleyJ., OwenR.J. and PounderR.E. (1992): Helicobacter pylori DNA patterns in bacteria isolated from gastric antrum, body and duodenum -Gastroenterol. 102: 829–833.
ScottN., LansdownM., DiamentR., RathboneB., MurdayV., WyattJ.I., McMahonM., DixonM.F. and QuirkeP. (1990): Helicobacter gastritis and intestinal metaplasia in a gastric cancer family - Lancet i: 728.
SimorA.E., ShamesB., DrummB.et al. (1990): Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile - J. Clin. Microbiol. 28: 83–86.
TaylorD.N. and BlaserM.J. (1991): The epidemiology of Helicobacter pylori infection -Epidemiol Rev. 13: 42–59.
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Owen, R.J., Desai, M., Figura, N. et al. Comparisons between degree of histological gastritis and DNA fingerprints, cytotoxicity and adhesivity of Helicobacter pylori from different gastric sites. Eur J Epidemiol 9, 315–321 (1993). https://doi.org/10.1007/BF00146270
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DOI: https://doi.org/10.1007/BF00146270