Summary
Ten hamsters received repeated injections of 3H-thymidine for 4 days and were allowed to survive for 7, 28, 42 and 100 days. Changes in spatial distribution of the labelled cells and in labelling indices of each cell line in the gastric glands were studied at various days after 3H-thymidine injections, and the fate of the mucous neck cell, the replacement of the chief cell and the mode of cell migration were discussed.
After 4 days of repeated injections of 3H-thymidine, the labelled parietal cells and the mucous neck cells were concentrated at the neck area. Starting from the neck area, they migrated an average of 3 micra downwards per day. By 42 days, they reached the middle level of the glands, where the labelled mucous neck cells decreased but the labelled chief cells increased in number. The differentiation of the chief cell then appears to take place at the middle level of the glands through transformation of the migratory mucous neck cells. After 4 days of the labelling, about 1.8% of the chief cells located in the lower part of the glands was found to undergo in situ replication. This indicates that the renewal of this cell type is partly assured by its own mitotic activity.
The foveolar cell — the future surface epithelium — seems to migrate upwards along the long axis of the glandular tubule in the pipe line system, which means “first produced, first migrates”. After migrating out from the neck area, the parietal cell and the mucous neck cell (the future chief cell) take an average of 200 days to reach the lower end of the glands. In the process of migration, however, the cells produced contemporaneously at the neck area became scatteringly spread from the neck towards the bottom of the gland. The time required for the newly-formed cells to reach the lower end of the gland varied between 100 and 300 days. In the gastric glands the cells first produced at the neck area do not first reach the lower end of the glands. This mode of random migration is referred to as the “stochastic flow system”. As one of the probable factors which disturb the pipe line flow of downward cell migration, cellular movements perpendicular to the long axis of the glandular tubule were suggested to occur at random at an any level of the gastric glands.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bensley, R.R.: Cowdry's special cytology, Vol. 1: The gastric glands, p. 197. New York: Paul B. Hoeber Inc. 1932
Corpron, R.E.: The ultrastructure of the gastric mucosa in normal and hypophysectomized rats. Amer. J. Anat. 118, 53–90 (1966)
Creamer, B., Shorter, R.G., Bamforth, J.: The turnover and shedding of epithelial cells. I. The turnover in the gastrointestinal tract. Gut 2, 110–118 (1961)
Eder, M., Rostock, H., Vogel, G.: Die Wirkung von Folsäureantagonisten auf die Regeneration der Darmschleimhaut. Virchows Arch. path. Anat. 341, 164–176 (1966)
Fujita, S., Shimada, M. Nakamura, T.: 3H-thymidine autoradiographic studies on the cell proliferation and the differentiation in the external and the internal granular layers of the mouse cerebellum. J. comp. Neurol. 128, 191–208 (1966)
Hattori, T.: On cell proliferation and differentiation of the gastric mucosa of the golden hamster. Cell Tiss. Res. 148, 213–226 (1974)
Hughes, W.L., Bond, V.P., Brecker, G., Cronkite, E.P., Painter, R.B., Quastler, H., Sherman, F.G.: Cellular proliferation in the mouse as revealed by autoradiography with tritiated thymidine. Proc. nat. Acad. Sci. (Wash.) 44, 476–483 (1958)
Hunt, T.E., Hunt, E.A.: Radioautographic study of proliferation in the stomach of the rat using thymidine-H3 and compound 48/80. Anat. Rec. 142, 505–517 (1962)
Iversen, O.H., Bjerkness, B., Devik, F.: Kinetics of cell renewal, cell migration and cell loss in the hair less mouse dorsal epidermis. Cell Tiss. Kinetics 1, 351–367 (1968)
Kaku, H.: Dynamic analysis of the stomach epithelium. Arch. histol. jap. 27, 223–246 (1966)
Kataoka, K.: Electron microscopic observations on cell proliferation and differentiation in the gastric mucosa of the mouse. Arch. histol. jap. 32, 251–273 (1970)
Lipkin, M., Sherlock, P., Bell, B.: Cell proliferation kinetics in the gastro-intestinal tract of man. II. Cell renewal in stomach, ileum, colon, rectum. Gastroenterology 45, 405–417 (1964)
MacDonald, W.C., Trier, J.S., Everett, N.B.: Cell proliferation and migration in the stomach, duodenum and rectum of man. Gastroenterology 46, 721–729 (1964)
Messier, B., Leblond, C.P.: Cell proliferation and migration as revealed by radioautography after injections of thymidine-H3 into male rats and mice. Amer. J. Anat. 106, 247–285 (1960)
Myrhe, E.: Regeneration of the fundic mucosa in rats. V. An autoradiographic study on the effect of cortisone. Arch. Path. 70, 476–485 (1960)
Oehlert, W., Büchner, T.: Mechanismus und zeitlicher Ablauf der physiologischen Regeneration im mehrschichtigen Plattenepithel und in der Schleimhaut des Magen-Darmtraktes der weißen Maus. Beitr. path. Anat. 125, 374–402 (1961)
Ragins, H., Winckze, F., Liu, S.M., Ditbrenner, H.: The origin and survival of the gastric parietal cells in the mouse. Anat. Rec. 162, 99–110 (1968)
Stevens, C.E., Leblond, C.P.: Renewal of the mucous cell in the gastric mucosa of the rat. Anat. Rec. 115, 231–245 (1953)
Willems, G., Galand, P., Vansteenkiste, Y., Zeitoun, P.: Cell population kinetics of zymogen and parietal cells in the stomach of mice. Z. Zellforsch. 134, 505–518 (1972)
Author information
Authors and Affiliations
Additional information
Supported by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan
Rights and permissions
About this article
Cite this article
Hattori, T., Fujita, S. Tritiated thymidine autoradiographic study on cellular migration in the gastric gland of the golden hamster. Cell Tissue Res. 172, 171–184 (1976). https://doi.org/10.1007/BF00226025
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00226025