Skip to main content
SpringerLink
Log in

The peri-insular acini of the pancreas of the rat

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

  1. 1.

    The cells of the peri-insular acini have larger nuclei and zymogen regions and are refractory to the secretion stimulating action of pilocarpine, in contrast to the cells of the common acini (the halo phenomenon).

  2. 2.

    In addition to the data in the literature suggesting a relation between the presence of insulin producing B-cells in the islets and the halo phenomenon, we have shown that the destruction of the B-cells by alloxan results in a disappearance of the haloes.

  3. 3.

    The peri-insular cells incorporate more leucine-3H than the cells of the common acini. The larger mass of ergastoplasm mainly accounts for this increased protein synthesis, the incorporation per unit volume of ergastoplasm not being substantially increased.

  4. 4.

    This great protein synthesis in cells maximally filled with zymogen granules is in accordance with our finding (Kramer and Poort, 1968) that the rate of protein synthesis in the cells of the rat pancreas is a constant one, independent of the phase of the secretory cycle. As in common acini, 90 per cent of the protein synthesized during 45 min, in the peri-insular acini of a “resting”, non stimulated gland is lost from the cells within 24 hours. As much protein must leave the cell as is synthesized in order to prevent overfilling.

  5. 5.

    A short discussion is given on the influence insulin can have on the secretion and on the protein synthesis in the acinar cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Coalson, R. E.: Pseudoisocyanin staining of insulin and specificity of empirical islet cell stains. Stain Technol. 41, 121–129 (1966).

    Google Scholar 

  • Daly, M. M., and A. E. Mirsky: Formation of protein in the pancreas. J. gen. Physiol. 36, 243–254 (1953).

    Google Scholar 

  • Ebels, I.: Some investigations concerning the disorder of glucose utilization in alloxan diabetes (English summary). Thesis, Utrecht, 1958.

  • Elsas, L. J., I. Albrecht, W. Koehne, and L. E. Rosenberg: Effect of puromycin on insulin-stimulated amino acid transport in muscle. Nature (Lond.) 214, 916–917 (1967).

    Google Scholar 

  • Ferner, H.: Die Dissemination der Hodenzwischenzellen und der Langerhansschen Inseln als funktionelles Prinzip für die Samenkanälchen und das exokrine Pankreas. Z. mikr.- anat. Forsch. 63, 35–51 (1958).

    Google Scholar 

  • —: Die orthologische Histologie und Cytologie der Langerhansschen Inseln. Verh. dtsch. Ges. Path. 42, 25–28 (1959).

    Google Scholar 

  • Hebb, C. O.: The effect of insulin administration on the response of the pancreas to parasympathic stimulation. Quart J. exp. Physiol. 26, 339–354 (1937).

    Google Scholar 

  • Hellman, B., A. Wallgren, and B. Petersson: Cytological characteristics of the pancreatic cells with regard to their position in relation to the islets of Langerhans. Acta endocr. (Kbh.) 39, 465–473 (1962).

    Google Scholar 

  • Hirsch, G. Chr.: Die Lebendbeobachtung der Restitution des Sekretes im Pankreas. Z. Zellforsch. 15, 36–68 and 290–310 (1932).

    Google Scholar 

  • Kramer, M. F., and C. Poort: To be published in this journal (1968).

  • Kurnick, N. B.: Pyronin Y in the methylgreen-pyronin histological stain. Stain Technol. 30, 213–230 (1955).

    Google Scholar 

  • Nagelschmidt, L.: Untersuchungen über die Langerhansschen Inseln der Bauchspeicheldrüse bei den Vögeln. Z. mikr.-anat. Forsch. 45, 200–232 (1939).

    Google Scholar 

  • Rampersad, O. R., and I. G. Wool: Protein synthesis by ribosomes from heart muscle: effect of insulin and diabetes. Science 149, 1102–1103 (1965).

    Google Scholar 

  • Sergeyeva, M. A.: Microscopic changes in the pancreatic gland of the cat produced by sympathetic and parasympathetic stimulation. Anat. Rec. 71, 319–336 (1938).

    Google Scholar 

  • Steiner, D. F., and J. King: Insulin-stimulated RNA synthesis and RNA polymerase activity in alloxan-diabetic liver. Biochim. biophys. Acta (Amst.) 119, 510–516 (1966).

    Google Scholar 

  • Wool, I. G., and Ph. Cavicchi: Insulin regulation of protein synthesis by muscle ribosomes: effect of the hormone on translation of messenger RNA for a regulatory protein. Proc. nat. Acad. Sci. (Wash.) 56, 991–998 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Histology, Faculty of Medicine, State University of Utrecht, The Netherlands

    M. F. Kramer & H. T. Tan

  2. Laboratorium voor Histologie, Nicolaas Beetsstraat 22, Utrecht 1, The Netherlands

    M. F. Kramer

Authors
  1. M. F. Kramer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. T. Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was carried out during a visit of Mr. H. T. Tan (Department of Histology, University of Makassar, Indonesia) to the Department of Histology of the University of Utrecht. This visit was made possible by a grant from the Netherlands Ministry of Foreign Affairs. Mr. Tan is grateful to the Staffs of the Ministry and of the Histological Department for their helpfulness and kindness.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kramer, M.F., Tan, H.T. The peri-insular acini of the pancreas of the rat. Zeitschrift für Zellforschung 86, 163–170 (1968). https://doi.org/10.1007/BF00348522

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00348522

Keywords

Search

Navigation