Abstract
The effects of motilin on gastrointestinal muscles show great variations in different organs and different species. For a precise regional differentiation, we recorded the mechanical activity of longitudinal and circular strips from fundus, corpus and antrum and of circular preparations from the inner and outer layer of the pyloric sphincter and from the duodenum (20 human stomachs). Motilin produced excitatory effects on the mechanical activity of the circular muscle strips from all regions of the human stomach including the pylorus. The effects on longitudinal preparations and on duodenal strips were weak. The most striking effect was an increase of phasic activity (amplitude) in circular antrum preparations, which exceeded the acetylcholine- and bombesin-induced activity. In pylorus preparations, a strong stimulation of phasic activity was observed with a transition to tonic activity in the inner layer of the pyloric ring at high motilin concentrations. The motilin-induced activity of the pyloric preparations was greater than the acetylcholine-induced contractions and even exceeded the bombesin-induced responses in the outer pylorus. The responses of the muscle strips of the proximal stomach (fundus and corpus) were weaker and did not exceed the acetylcholine-induced activity. All effects remained unaltered by atropine and tetrodotoxin application. The study confirms that motilin can interact directly with the smooth muscle of human stomach.
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Achem-Karam SR, Funakoshi A, Vinik AI, Owyang C (1985) Plasma motilin concentration and interdigestive migrating motor complex in diabetic gastroparesis: Effect of metocloprymide. Gastroenterology 88:492–499
Adachi H, Toda N, Hyashi S, Noguchi M, Suzuki T, Torizuka K, Yajima H Koyama K (1981) Mechanisms of the excitatory action of motilin on isolated rabbit intestine. Gastroenterology 80:783–788
Bloom SR, Mitznegg P, Bryant MG (1976) Measurement of human plasma motilin. Scand J Gastroenterol 11 (Suppl 39):47–52
Bloom SR, Christofides ND, Modlin J, Fitzpatrick ML (1978) Effect of motilin on gastric emptying of solid meals in man. Gastroenterology 74:A 1010
Bormans V, Peeters TL, Vantrappen G (1986) Motilin receptors in rabbit stomach and small intestine. Regul Pept 15:143–153
Brown JC, Mutt V, Dryburgh JR (1971) The further purification of motilin, a gastric motor activity stimulating polypeptide from the mucosa of the small intestine of hogs. Can J Physiol Pharmacol 49:399–405
Chey WY, Lee KY (1980) Actions of motilin on gastrointestinal motility and plasma immunoreactive motilin concentrations in interdigestive and postprandial states. Endocrinol Jpn 1:173–177
Christofides ND, Modlin JM, Fitzpatrick ML, Bloom SR (1979) Effect of motilin on the rate of gastric emptying and gut hormone release during breakfast. Gastroenterology 76:903–907
Domschke W, Strunz U, Mitznegg P, Ruppin H, Domschke S, Schubert E, Wünsch E, Jaeger E, Demling L (1974) 13-Norleucin-Motilin — Analyse der Wirkungen auf den Gastrointestinaltrakt. Naturwissenschaften 61:370
Fox JET, Daniel EE, Jury J, Fox AE, Collins AM (1983) Sites and mechanisms of action of neuropeptides on canine gastric motility in vivo and in vitro. Life Sci 33:817–825
Funakoshi A, Glowniak J, Owyang C, Vinik AI (1982) Evidence for cholinergic and vagal noncholinergic mechanisms modulating plasma motilin-like immunoreactivity. J Clin Endocrinol Metab 54:1129–1134
Helmstaedter V, Kreppein W, Domschke W, Mitzegg P, Yanaihara N, Wünsch E, Forssmann WG (1979) Immunohistochemical localizations of motilin in endocrine nonenterochromaffin cells of the small intestine of humans and monkey. Gastroenterology 76:897–902
Itoh Z, Sekiguchi T (1983) Interdigestive motor activity in health and disease. Scand J Gastroenterol 82:121–134
Itoh Z, Honda R, Hiwatashi K, Takeuchi S, Aizawa R, Takayanagi R, Couch EF (1976) Motilin-induced mechanical activity in the canine alimentary tract. Scand J Gastroenterol 11 (Suppl 39):93–110
Janssens J, Vantrappen G, Peeters TL (1983) The activity front of migrating motor complex of the human stomach but not of the small intestine is motolin-dependent. Regul Pept 6:363–369
Jennewein HM, Hummelt H, Siewert R, Waldeck, F (1975) The motor-stimulating effect of natural motilin on the lower esophageal sphincter, fundus, antrum and duodenum in dogs. Digestion 13:246–250
Jenssen TG, Burhol PG, Jorde R, Florholmen J, Lygren I (1984) Radioimmunoassayable plasma motilin in man. Scand J Gastroenterol 19:717–723
Labò G, Bortolotti M, Vezzadini P, Bonora G, Bersani G (1986) Interdigestive gastroduodenal motility and serum motilin levels in patients with idiopathic delay in gastric emptying. Gastroenterology 90:20–26
Lee KY, Chang TM, Chey WY (1983) of rabbit anti-motilin serum on myoelectric activity and plasma motilin concentration in fasting dog. Am J Physiol 245:G 547-G 553
Louie D, Owyang C (1986) Motilin receptors on isolated gastric smooth muscle cells: interaction with acetylcholine. Gastroenterology 86:A 206
Lüdtke FE, Golenhofen K, Becker HD (1984) Mechanical activity of isolated human pyloric muscle, in comparison with canine pylorus. In: Roman C (ed) Gastrointestinal motility, MTP Press Ltd, Lancaster, pp 84–94
Owyang C, Funakoshi A, Vinik AI (1983) Evidence for modulation of motilin secretion by pancreatico-bilary juice in health and chronic pancreatitis. J Clin Endocrinol Metabol 57:1015–1020
Pearse AGE, Polak JM, Bloom SR, Adams C, Dryburgh JR, Brown JC (1974) Enterochromaffin cells of the mammalian small intestine as the source of motilin. Virchows Arch [Cell Pathol] 16:111–120
Peeters TL, Vantrappen G, Janssens J (1980) Fasting plasma motilin levels are related to the interdigestive motility complex. Gastroenterology 79:716–719
Segawa T, Nakano M, Kai Y, Kawatani H, Yajima H (1976) Effect of synthetic motilin and related polypeptides on contraction of gastrointestinal smooth muscle. J Pharm Pharmacol 28:650–651
Schulze-Delrieu K, Shirazi SS (1983) Neuromuscular differentiation of the human pylorus. Gastroenterology 84:287–292
Strunz U, Domschke W, Mitznegg P, Domschke S, Schubert E, Wünsch E, Jaeger E, Demling L (1975) Analysis of the motor effects of 13-norleucine-motilin on the rabbit, guinea pig, rat, and human alimentary tract in vitro. Gastroenterology 68:1485–1491
Vantrappen G, Janssens J, Peeters TL, Bloom SR, Christofides ND, Hellemans J (1979) Motilin and the interdigestive migrating motor complex in man. Dig Dis Sci 24:497–500
You CH, Chey WY, Lee KY (1980) Studies on plasma motilin concentration and interdigestive motility of the duodenum in humans. Gastroenterology 79:62–66
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Lüdtke, F.E., Müller, H. & Golenhofen, K. Direct effects of motilin on isolated smooth muscle from various regions of the human stomach. Pflugers Arch. 414, 558–563 (1989). https://doi.org/10.1007/BF00580991
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DOI: https://doi.org/10.1007/BF00580991