Summary
Effects of diet, hibernation and seasonal variations on hydrolase activities were determined in mucosa and purified brush border membranes of the small intestine of European hamsters. Wild hamsters captured in April and fed for several weeks with an equilibrated laboratory chow (20% protein, 50% carbohydrates) exhibited a rise in disaccharidase activities (sucrase, isomaltase, lactase) but no changes in aminopeptidase N activity. During deep hibernation, in contrast to sucrase and isomaltase activities which showed only minor changes, lactase activity was significantly enhanced along the jejunoileum, and aminopeptidase N activity was maximum in the ileum. After a short period (48 h) of wakefulness and feeding following 10 days of starvation during the hibernation period, the activities of the disaccharidases and of aminopeptidase N returned to values measured in active animals. In contrast to the nutritional state, which has an important impact on the activities of intestinal enzymes, season has little effect on the intestine of the active animal under a controlled environment. The pattern of enzyme activities which occurs along the small intestine in the hibernating animal may be a prerequisite for optimum digestion during the short phases of waking during the hibernation period of the European hamster.
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Bustamante S, Gasparo M, Kendall K, Coates P, Brown S, Somawane B, Koldovsky O (1981) Increased activity of rat intestinal lactase due to increased intake of α-saccharides (starch, sucrose) in isocaloric diets. J Nutr 111:943–953
Canguilhem B, Vivien-Roels B, Demeneix B, Miro JL, Masson-Pevet M, Pevet P (1986) Seasonal and endogenous variations of plasma testosterone (T), thyroxine (T4) and triiodothyronine (T3) in the European hamster. In: Assenmacher I, Boissin J (eds) Endocrine regulations as adaptive mechanisms to environment. Editions du CNRS, Paris, pp 291–295
Csàky TZ, Gallucci E (1977) Seasonal orientation in the active transporting ability and in the membrane ATPase activity of the frog intestinal epithelium. Biochim Biophys Acta 466:521–525
Dahlqvist A (1964) Method for assay of intestinal disaccharidases. Anal Biochem 7:18–25
Deren JJ, Broitman SA, Zamchek N (1967) Effect of diet upon intestinal disaccharidases and disaccharide absorption. J Clin Invest 46:186–195
Goodman MN, Larsen PR, Kaplan MM, Aoki TT, Young VR, Ruderman NB (1980) Starvation in the rat. II. Effect of age and obesity on protein sparing and fuel metabolism. Am J Physiol 239:277–286
Karasov WH, Diamond JM (1983) Adaptive regulation of sugar and amino-acid transport by vertebrate intestine. Am J Physiol 245:G443-G462
Koldovsky O, Asp NG, Dahlqvist A (1969) A method for the separate assay of ‘neutral’ and ‘acid’ B-galactosidase in homogenates of rat small intestinal mucosa. Anal Biochem 27:409–418
Latif SA, Zain BK, Zain-Ul-Abedin M (1967) Intestinal transport of sugars in a lizard during hibernation and activity. Comp Biochem Physiol 23:121–128
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Maroux S, Louvard D, Baratti J (1973) The aminopeptidase from hog intestinal brush border. Biochim Biophys Acta 321:282–295
McCarthy DM, Nicholson JA, Kim YS (1980) Intestinal enzyme adaptation to normal diets of different composition. Am J Physiol 239:G6445-G6451
McNeill LK, Hamilton JR (1971) The effect of fasting on disaccharidase activity in the rat small intestine. Pediatrics 47:65–72
Montoya R, Ambid L, Agid R (1979) Torpor induced at any season by suppression of food proteins in a hibernator, the garden dormouse (Eliomys quercinus L.). Comp Biochem Physiol 62A:371–376
Musacchia XJ, Westhoff DD (1964) Absorption of D-glucose by segments of intestine from active and hibernating, irradiated and nonirradiated ground squirrels,Citellus tridecemlinaetus. Am Acad Sci Fenn Ser Au 71:345–359
Nsi-Emvo E, Launay JF, Raul F (1987) Is adult-type hypolactasia in the intestine of mammals related to changes in the intracellular processing of lactase? Cell Mol Biol 33:335–344
Quadri M, Zain BK, Zain-Ul-Abedin (1970) Intestinal transport of amino-acids in a lizard during hibernation and activity. Comp Biochem Physiol 36:569–577
Raul F, Simon PM, Kedinger M, Grenier JF, Haffen K (1980) Effect of sucrose refeeding on disaccharidase and aminopeptidase activities of intestinal villus and crypt cells in adult rats. Evidence for a sucrose dependent induction of sucrase in the crypt cells. Biochim Biophys Acta 630:1–9
Raul F, Doffoel M, Marescaux J, Bockel R, Grenier JF (1982a) Effects of prolonged alcohol administration and a high carbohydrate-low protein diet on the activities of the jejunal brush border enzymes in the rat. Gut 23:962–967
Raul F, Noriega R, Doffoel M, Grenier JF, Haffen K (1982b) Modifications of brush border enzyme activities during starvation in the jejunum and ileum of adult rats. Enzyme 28:328–335
Raul F, Noriega R, Nsi-Emvo E, Doffoel M, Grenier JF (1983) Lactase activity is under hormonal control in the intestine of adult rat. Gut 24:648–652
Raul F, Gosse F, Goda T, Koldovsky O (1987) Short-term effect of a high-protein low carbohydrate diet on aminopeptidase in adult rat jejunoileum. Site of aminopeptidase response. Biochem J 247:401–405
Rosensweig NS, Herman RM (1969) Time response of jejunal sucrase and maltase activity to a high sucrose diet in normal man. Gastroenterology 56:500–505
Sauerbier I, Von Mayersbach H (1976) Circadianrhythmischhistochemische Untersuchungen am Dünndarmepithel von Winterschläfern. Acta Histochem 16:155–160
Schmitz J, Preiser H, Maestracci D, Ghosh BK, Cerda JJ, Crane RK (1973) Purification of the human intestinal brush border membrane. Biochim Biophys Acta 92:305–311
Soria-Milla MA (1986) Structure of the mucosa of the small intestine proximal portion in the hibernating garden dormouse (Eliomys quercinus L.). Cryo Letters 7:184–193
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Galluser, M., Raul, F. & Canguilhem, B. Adaptation of intestinal enzymes to seasonal and dietary changes in a hibernator: the European hamster (Cricetus cricetus). J Comp Physiol B 158, 143–149 (1988). https://doi.org/10.1007/BF01075827
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DOI: https://doi.org/10.1007/BF01075827