Abstract
The effect on parietal cells of glucagon given prior to pentagastrin is unknown. Fifteen healthy volunteers were studied during constant intravenous infusion of pentagastrin (2μg/kg body weight/hr) and during pentagastrin infusion initiated 20 min after intravenous injection of 2 mg glucagon. Three types of studies were performed: Gastric mucosal biopsies were obtained with a Quinton instrument. Electron micrographs of 320 parietal cells were analyzed by the Loud quantitative method, and intragastric pH (pH probe), or gastric potential difference (PD) were recorded continuously. Pentagastrin infusion produced a significant increase in canalicular and simultaneous reduction of tubulovesicular membrane area of parietal cells. Glucagon pretreatment did not inhibit canalicular and tubulovesicular membrane reaction to pentagastrin; unexpectedly this reaction was significantly greater when compared to that after pentagastrin alone. Initiation of pentagastrin infusion in subjects pretreated with glucagon produced a greater absolute value drop in gastric PD (23±2mV) and an earlier drop in intragastric pH (3 min) than in subjects receiving pentagastrin infusion alone (13±1mV and 10min, respectively). In conclusion, at the doses studied, glucagon pretreatment increases parietal cell canalicular reaction to pentagastrin.
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References
Dotevall G, Kock NG, Walan A: Inhibition of pentagastrin-induced gastric acid secretion in man by glucagon given intravenously. Scand J Gastroenterol 4:713–716, 1969
Christiansen J, Hendel L: The effect of glucagon on pentagastrin-induced gastric acid secretion and serum calcium concentration in man. Scand J Gastroenterol 8:81–84, 1973
Christiansen J, Hendel L: The effect of glucagon on pentagastrin-induced gastric secretion of sodium, potassium, and calcium in man. Scand J Gastroenterol 9:437–440, 1974
Christiansen J, Holst J, Kalaja E: Inhibition of gastric acid secretion in man by exogenous and endogenous pancreatic glucagon. Gastroenterology 70:688–692, 1976
Konturek S, Biernat J, Kwiecien N, Oleksy J: Effect of glucagon on meal-induced gastric secretion in man. Gastroenterology 68:448–454, 1975
Charbon G, Sebus J, Kool D, Hoekstra M: Augmentation by glucagon of histamine-induced gastric secretion. Gastroenterology 44:805–810, 1963
Frexinos J, Carballido M, Louis A, Ribet A: Effects of pentagastrin stimulation on human parietal cells. An electron microscope study with quantitative evaluation of cytoplasmic structures. Am J Dig Dis 16:1065–1074, 1971
Zalewsky C, Moody F: Stereological analysis of the parietal cell during acid secretion and inhibition. Gastroenterology 73:66–74, 1977
Helander H, Hirschowitz B: Quantitative ultrastructural studies on gastric parietal cells. Gastroenterology 63:951–961, 1972
Hamer D, Price A, Baron J: Morphological and pharmacological studies of the parietal cells of the stomach in the dog during periods of maximal acid output and after gastric secretory inhibitor UK-9040. Gut 18:91–98, 1977
Ivey KJ, Tarnawski A, Sherman D, Krause WJ, Ackman K, Burks M, Hewitt J: Quantitative ultrastructural analysis of the human parietal cell during acid inhibition and elevation of gastric potential difference by glucagon. Gut 21:3–8, 1980
Tarnawski A, Ivey KJ, McGuigan JE, England J: Effect of glucagon and pentagastrin on gastric mucosal potential difference in man. Gut 19:1116–1120, 1978
Tarnawski A, Krause WJ, Ivey KJ: Effect of glucagon on aspirin-induced gastric mucosal damage in man. Gastroenterology 74:240–245, 1978
Baskin W, Ivey KJ, Krause WJ, Jeffrey G, Gemmell R: Aspirin-induced ultrastructural changes in human gastric mucosa. Ann Intern Med 85:299–303, 1976
Luft J: Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414, 1961
Spurr A: A low viscosity epoxy resin embedding medicine for electron microscopy. J Ultrastruc Res 26:31–35, 1969
Morozov I.: Ultrastructural topography of parietal cells of the gastric mucosa (morphometric investigation). Bull Exp Biol Med 82:1740–1743, 1976
Loud A: A method for the quantitative estimation of cytoplasmic structures. J Cell Biol 15:481–487, 1962
Loud A, Baranu W, Pack B: Quantitative evaluation of cytoplasmic structures in electron micrographs. Lab Invest 14:996–1008, 1965
Ivey KJ, Baskin W, Jeffrey G: Effect of cimetidine on gastric potential difference in man. Lancet 2:1972–1973, 1975
Snedecor G, Cochran W: Statistical Methods, 6th ed. Ames, Iowa State University Press, 1967
Leeson TS: Canaliculi and tubulovesicles of rat parietal cells. Am J Anat 136:541–547, 1973
Aadland E, Berstad A, Granerus G: Augmented parietal cell sensitivity to histamine after cimetidine treatment in healthy subjects. Scand J Gastroenterol 16:87–91, 1981
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This work was supported in part by the Medical Research Service of the Veterans Administration.
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Ivey, K.J., Tarnawski, A., Krause, W.J. et al. Effect of pentagastrin on parietal cell ultrastructure in glucagon-pretreated subjects. Digest Dis Sci 27, 394–400 (1982). https://doi.org/10.1007/BF01295646
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DOI: https://doi.org/10.1007/BF01295646