Abstract
To examine the roles of histamine and diamine oxidase in the intestine after ischemiareperfusion, we measured histamine content, diamine oxidase activity, and ornithine decarboxylase activity in rat intestinal mucosa 6 hr following various periods of ischemia. In addition, mortality rates of rats after various periods of ischemia were observed. The superior mesenteric artery was occluded for 15, 30, or 60 min. Ornithine decarboxylase activity increased in the 15-, 30-, and 60-min ischemic groups compared to the shamoperated control group. In the prolonged ischemic group (60-min ischemia), both histamine concentration and diamine oxidase activity in the mucosa decreased, contributing to an increase in circulating histamine. In the 60-min ischemic group, the mortality rate of rats was 25%, which was significantly larger than the control groups. Pretreatment with aminoguanidine, which suppressed diamine oxidase activity, increased the mortality rate. These results indicate that histamine released from the intestinal mucosa has a harmful effect on rats, and diamine oxidase activity plays an important role when the small intestine is subjected to prolonged period of ischemia.
Similar content being viewed by others
References
Fujimoto K, Imamura I, Granger DN, Wada H, Sakata T, Tso P: Histamine and histidine decarboxylase are correlated with mucosal repair in rat small intestine after ischemiareperfusion. J Clin Invest 89:126–133, 1992
Johnson LR: Regulation of gastrointestinal mucosal growth. Physiol Rev 68:456–502, 1988
Fujimoto K, Granger DN, Price VH, Tso P: Ornithinedecarboxylase is involved in repair of small intestine after ischemia-reperfusion in rats. Am J Physiol 261:G523-G529, 1991
Fujimoto K, Price VH, Granger DN, Specian R, Bergstedt S, Tso P: Effect of ischemia-reperfusion on lipid digestion and absorption in rat intestine. Am J Physiol 260:G595-G603, 1991
Kusche J, Lorenz W, Stahlknecht CD, Richter H, Hesterberg R, Schmal A, Hinterlang E, Weber D, Ohmann C: Intestinal diamine oxidase and histamine release in rabbit mesenteric ischemia. Gastroenterology 80:780–787, 1981
Tsuruta Y, Kokashi K, Ohkura Y: Simultaneous determination of histamine andN-methyl histamine in human and rat brain by high-performance liquid chromatography with fluorescence determination. J Chromatogr 224:105–110, 1981
Oishi R, Itoh Y, Nishibori M, Saeki K: Feeding-related circadian variation in telemethylhistamine levels of mouse and rat brains. J Neurochem 49:541–547, 1987
Kusche J, Lorenz W: Diamine oxidase.In Methods of Enzymatic Analysis, (3rd ed.), Vol 3. HU Bergmeyer (ed). Weinheim, Verlag, Chemie, 1983, pp 237–250
Tabata K, Johnson RJ: Ornithine decarboxylase and mucosal growth in response to feeding. Am J Physiol 251:G270-G274, 1986
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951
Kim KS, Backus B, Harris M, Rourke P: Distribution of diamine oxidase and imidazole-N-methyltransferase along the gastrointestinal tract. Comp Biochem Physiol 31:137–145, 1969
Baylin SB, Stevens SA, Shakir KMM: Association of diamine oxidase and ornithine decarboxylase with maturing cells in rapidly proliferating epithelium. Biochim Biophys Acta 541:415–419, 1978
Luk GD, Marton LJ, Baylin SB: Ornithine decarboxylase is important in intestinal mucosal maturation and recovery from injured rats. Science 210:195–198, 1980
Luk GD, Bayless TM, Baylin SB: Diamine oxidase (histaminase) a circulating marker for rat intestinal mucosal maturation and integrity. J Clin Invest 66:66–70, 1980
Granger DN: Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury. Am J Physiol 255:H1269-H1275, 1988
Author information
Authors and Affiliations
Additional information
Grant support: Grant-in-Aid from the Ministry Education in Japan for Scientific Research 04770423, and Uehara Memorial Foundation.
Rights and permissions
About this article
Cite this article
Fujisaki, J., Fujimoto, K., Oohara, A. et al. Roles of histamine and diamine oxidase in mucosa of rat small intestine after ischemia-reperfusion. Digest Dis Sci 38, 1195–1200 (1993). https://doi.org/10.1007/BF01296067
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01296067