Abstract
Altered intestinal motility and diarrhea are features of food protein-induced intestinal anaphylaxis in the conscious rat. These experiments were performed to determine the mediator(s) responsible for jejunal circular smooth muscle contraction during this response. Hooded-Lister rats were sensitized by intraperitoneal injection of 10-μg egg albumin, and controls were sham-sensitized with saline. Fourteen days later the contractility of the circular muscle in jejunal segments (mucosa intact) was examined in standard tissue baths in response to antigen (Ag) or other agents. While control and sensitized tissues contracted in similar fashion in response to stretch, bethanechol, histamine, or 5-hydroxytryptamine (5HT), Ag contracted only the segments of sensitized animals. The contractile response was: (1) specific to the sensitizing Ag, as bovine serum albumin did not induced contraction and (2) could be passively transferred with serum containing specific immunoglobulin E antibody (IgE-Ab). Concanavalin A, which degranulates both mucosal and connective tissue-type mast cells, and compound 48/80, which degranulates only connective tissue-type mast cells produced contractile responses. Ag-induced contraction was significantly inhibited by the mucosal and connective tissue-type mast cell stabilizer doxantrazole, but not the connective tissue mast cell stabilizer disodium cromoglycate. Diphenhydramine and cimetidine together significantly inhibited histamine-induced contraction, but failed to effect the Ag-induced contraction in sensitized tissues. While the contractile response to 5HT was reduced in the presence of methysergide (5HT1-receptor antagonist), cinanserin (5HT2-receptor antagonist), and ICS 205-930 (5HT3-receptor antagonist), only cinanserin significantly inhibited the contractile response to Ag. Indomethacin significantly inhibited Ag-induced contraction. Ag-induced contraction was resistant to atropine and tetrodotoxin. Thus, food protein-induced alterations in intestinal motility in sensitized rats are due in part to an IgE-mediated mast cell degranulation, 5HT release, prostaglandin synthesis, and contraction of the circular smooth muscle.
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References
Ferguson A: Models of intestinal hypersensitivity. Clin Gastroenterol 5:271–288, 1976
Bock SA: Food sensitivity: A critical review and practical approach. Am J Dis Child 134:973–982, 1980
Scott RB, Diamant SC, Gall DG: The motility effects of IgE-mediated intestinal anaphylaxis in the rat. Am J Physiol 255:G505-G511, 1988
Diamant SC, Gall DG, Scott RB: The effect of intestinal anaphylaxis on postprandial motility in the rat. Can J Physiol Pharmacol 67:1326–1330, 1989
Maric M, Gall DG, Scott RB: The effect of IgE-mediated intestinal anaphylaxis on intestinal transit. Can J Physiol Pharmacol 67:1437–1441, 1989
Scott RB, Gall DG, Maric M: Mediation of food proteininduced jejunal smooth muscle contraction in sensitized rats. Am J Physiol 259:G6-G14, 1990
Perdue MH, Chung M, Gall DG: Effect of intestinal anaphylaxis on gut function in the rat. Gastroenterology 86:391–397, 1984
Meddings JB, Scott RB, Fick GH: Analysis and comparison of sigmoidal curves: Application to dose-response data. Am J Physiol 257:G982-G989, 1989
Fargeas MJ, Theodourou V, Fioramonti J, Bueno L: Relationship between mast cell degranulation and jejunal myelectric alterations in intestinal anaphylaxis in rats. Gastroenterology 102:157–162, 1992
Augustin R: Techniques for the study and assay of reagins in allergic subjects.In Handbook for Experimental Immunology. DM Weir (ed). London, Blackwell, 1979, pp 45.1–45.64
Marom Z, Casale TB: Mast cells and their mediators. Ann Allergy 50:367–370, 1983
Metcalfe DD: Mast cell mediators with emphasis on intestinal mast cells. Ann Allergy 53:563–575, 1984
Scott RB, Maric M: A limited role for leukotrienes and platelet activating factor in food protein-induced jejunal smooth muscle contraction in sensitized rats. Can J Physiol Pharmacol 69:1841–1846, 1991
Weisbrodt NW: Motility of the small intestine.In Physiology of the Gastrointestinal Tract, 2nd ed. LR Johnson (ed). New York, Raven Press, 1987, pp 631–633
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Scott, R.B., Maric, M. Mediation of anaphylaxis-induced jejunal circular smooth muscle contraction in rats. Digest Dis Sci 38, 396–402 (1993). https://doi.org/10.1007/BF01316490
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DOI: https://doi.org/10.1007/BF01316490