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The intratracheal administration of endotoxin: X. Dexamethasone downregulates neutrophil emigration and cytokine expression in vivo

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Abstract

Intratracheal instillation of endotoxin (LPS) causes acute pulmonary inflammation characterized by the accumulation of plasma proteins and leukocytes within the pulmonary airways. The synthetic glucocorticoid dexamethasone 1) inhibits the LPS-initiated vascular leak of plasma proteins into the airspace, 2) inhibits the LPS-initiated emigration of neutrophils and lymphocytes into the airspace in a dose-dependent fashion, and 3) inhibits LPS-initiated mRNA and/or bronchoalveolar lavage protein expression of cytokines (TNF, IL-1 and IL-6) and chemokines (MIP-lα, MIP-2 and MCP-1). In conclusion, dexamethasone inhibits both the vascular and cellular aspects of acute inflammation by downregulation of a broad spectrum of inflammatory cytokines and chemokines.

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Authors and Affiliations

  1. Department of Pathology, University of California at San Diego School of Medicine, 92103, San Diego, California

    Eunhee S. Yi, Adriana Bedoya & Thomas R. Ulich

  2. Department of Pathology, University of Michigan School of Medicine, 48109, Ann Arbor, Michigan

    Daniel G. Remick

  3. Laboratory of Molecular Immunoregulation Biological Response Modifiers Program, National Cancer Institute, 21702, Frederick, Maryland

    Young Lim

  4. Amgen, Inc., 1840 DeHavilland Drive, 91320, Thousand Oaks, California

    Winson Tang, Songmei Yin & Thomas R. Ulich

  5. Department of Pathology, University of California at Irvine School of Medicine, 92717, Irvine, California

    Christine E. Nadzienko

Authors
  1. Eunhee S. Yi
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  2. Daniel G. Remick
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  3. Young Lim
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  4. Winson Tang
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  5. Christine E. Nadzienko
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  6. Adriana Bedoya
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  7. Songmei Yin
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  8. Thomas R. Ulich
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Yi, E.S., Remick, D.G., Lim, Y. et al. The intratracheal administration of endotoxin: X. Dexamethasone downregulates neutrophil emigration and cytokine expression in vivo. Inflammation 20, 165–175 (1996). https://doi.org/10.1007/BF01487403

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  • DOI: https://doi.org/10.1007/BF01487403

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