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Changes in serum and intestinal diamine oxidase (DAO) activity after proximal enterectomy in rats

Correlation of DAO activity with mucosal mass parameters

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Abstract

To determine whether serum and mucosal DAO activity reflects quantitative changes in the small bowel mucosal mass, we have chosen an experimental model of mucosal hyperplasia which is known to occur in the rat after enterectomy. A 50% proximal enterectomy or a single transection was performed in 20 growing rats weighing 145–160 g. Ten days following surgery, we determined mucosal mass parameters (weight, protein, and DNA content), sucrase activity, and DAO activity in the duodenum (segment A), proximal ileum (segment B), and distal ileum (segment C) of the remaining small intestine. Mucosal hyperplasia was demonstrated by the finding that in each segment, mucosal weight, protein, and DNA content per centimeter of gut length were significantly (P<0.01) higher (+38 to+78%) in the resected group than in transected controls. In segments B and C of resected rats, the changes in DAO activity expressed per gram of mucosa paralleled the changes in mucosal mass, the activity being increased by +69% and +49% (P<0.05) compared to the values recorded in transected controls. Expressed per centimeter of gut length, total DAO activity was also enhanced by +141% in segment B (P<0.05 vs controls) and by +87% in segment C(P>0.01 vs controls) of resected rats. In the duodenum, the changes in DAO activity were small (+36%) and not significant. In the ileum (segment C), significant correlations were established between total DAO activity and either mucosal weight (r=0.75,N=20,P<0.01) or mucosal DNA concentration (r=0.78, N=20, P<0.01) per centimeter of gut length, but there was no correlation between DAO activity and sucrase activity. Compared to control rats with transection, proximal enterectomy produced marked changes in the serum activity of DAO. Ten days following surgery, the mean value of serum DAO was fivefold higher (P< it0.005) in the resected group than in the transected group. These data indicate that after jejunectomy (1) the intestinal activity of DAO reflects accurately quantitative changes of the mucosal mass in the remaining ileum but not in the duodenum, and (2) circulating levels of DAO could be used as a marker of ileal mucosal adaptation after proximal enterectomy.

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Authors and Affiliations

  1. Laboratory of Pediatric Gastroenterology and Nutrition, Unit of Gastroenterology, Faculty of Medicine, University of Louvain, Brussels, Belgium

    Jean -Paul Buts MD, Stéphane Theys, Nadine De Keyser & Charles Dive MD

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  1. Jean -Paul Buts MD
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  2. Stéphane Theys
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  3. Nadine De Keyser
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  4. Charles Dive MD
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Buts, J.P., Theys, S., De Keyser, N. et al. Changes in serum and intestinal diamine oxidase (DAO) activity after proximal enterectomy in rats. Digest Dis Sci 34, 1393–1398 (1989). https://doi.org/10.1007/BF01538075

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  • DOI: https://doi.org/10.1007/BF01538075

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