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Effect of iron on neonatal gut flora during the first three months of life

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Abstract

To study the effect of milk supplemented with iron on neonatal gut flora, faecal specimens of ten infants receiving breast milk, six receiving a cow-milk preparation supplemented with iron (5 mg/l) and seven receiving the same product without iron supplement (iron concentration <0.5 mg/l) were examined during the first 12 weeks of life. In breast-fed infants bifidobacteria was predominant, counts ofEscherichia coli were low, and other bacteria were rarely present. Infants receiving fortified cow-milk preparation had high counts ofEscherichia coli, counts and isolation frequency of bifidobacteria were low and other bacteria were frequently isolated. In those on unfortified cow-milk preparation isolation frequency ofEscherichia coli, bifidobacteria and bacteroides was comparable with that in breast-fed infants; however, counts ofEscherichia coli were high. It is concluded that the faecal flora of infants fed unfortified cow-milk preparation acquires characteristics of that found in breast-fed infants.

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References

  1. Ross, C. A. C., Dawes, E. A.: Resistance of the breastfed to gastroenteritis. Lancet 1954, i: 994–998.

    Google Scholar 

  2. Alexander, M. B.: Infantile diarrhoea and vomiting. A review of 456 infants in a hospital unit for enteritis. British Medical Journal 1948, ii: 973–978.

    Google Scholar 

  3. Bullen, C. L., Willis, A. T.: Resistance of the breast-fed to gastroenteritis. British Medical Journal 1971, iii: 338–343.

    Google Scholar 

  4. Bullen, C. L., Tearle, P. V.: Bifidobacteria in the intestinal tract of infants: an in-vitro study. Journal of Medical Microbiology 1976, 9: 335–344.

    PubMed  Google Scholar 

  5. Bullen, J. J., Rogers, H. J., Leigh, L.: Iron-binding proteins in milk and resistance toEscherichia coli infection in infants. British Medical Journal 1972, i: 69–75.

    Google Scholar 

  6. Rogers, H. J., Synge, C.: Bacteriostatic effect of human milk onEscherichia coli: the role of IgA. Immunology 1978, 34: 19–28.

    PubMed  Google Scholar 

  7. Neilands, J. B.: Iron and its role in microbial physiology. In: Neilands, J. B. (ed.): Microbial iron metabolism. Academic Press, New York, 1974, p. 3–34.

    Google Scholar 

  8. Bullen, J. J., Rogers, H. J., Griffiths, E.: Role of iron in bacterial infection. Current Topics in Microbiology and Immunology 1978, 80: 1–35.

    PubMed  Google Scholar 

  9. Weinberg, E. D.: Iron and infection. Microbiological Reviews 1978, 42: 45–66.

    Google Scholar 

  10. Mevissen-Verhage, E. A. E., Marcelis, J. H., Harmsen-Van Amerongen, W. C. M., De Vos, N. M., Berkel, J., Verhoef, J.: Effect of iron on development of the neonatal gut flora during the first week of life. European Journal of Clinical Microbiology 1985, 4: 14–18.

    PubMed  Google Scholar 

  11. Van der Wiel-Korstanje, J. A. A., Winkler, K. C.: Medium for differential count of the anaerobic flora in human faeces. Applied Microbiology 1970, 20: 168–169.

    PubMed  Google Scholar 

  12. Miles, A. A., Misra, S. S., Irwin, J. O.: The estimation of bactericidal power of the blood. Journal of Hygiene 1938, 38: 732–750.

    Google Scholar 

  13. Albert, M. J., Bhat, P., Maiya, P. P., Pereira, S. M., Baker, S. J.: Faecal flora of South Indian infants and young children in health and with acute gastroenteritis. Journal of Medical Microbiology 1978, 11: 137–143.

    PubMed  Google Scholar 

  14. Mata, L. J., Urrutia, J. J.: Intestinal colonization of breast-fed children in a rural area of low socio-economic level. Annals of the New York Academy of Science 1971, 176: 93.

    Google Scholar 

  15. Willis, A. T., Bullen, C. L., Williams, K., Fagg, C. G., Bourne, A., Vignon, M.: Breast milk substitute: a bacteriological study. British Medical Journal 1973, iv: 67–72.

    Google Scholar 

  16. Bullen, C. L., Tearle, P. V., Willis, A. T.: Bifidobacteria in the intestinal tract of infants: an in vivo study. Journal of Medical Microbiology 1976, 9: 325–333.

    PubMed  Google Scholar 

  17. Haenel, H.: Some rules in the ecology of the intestinal microflora of man. Journal of Applied Bacteriology 1961, 24: 242–251.

    Google Scholar 

  18. Bullen, C. L., Tearle, P. V., Stewart, M. G.: The effect of “humanized” milks and supplemented breast feeding on the faecal flora of infants. Journal of Medical Microbiology 1977, 10: 403–413.

    PubMed  Google Scholar 

  19. Mitsuoka, T.: Recent trends in research on intestinal flora. Bifidobacteria and Microflora 1982, 1: 3–24.

    Google Scholar 

  20. Rogers, H. J.: Iron-binding catechols and virulence inEscherichia coli. Infection and Immunity 1973, 7: 445–456.

    Google Scholar 

  21. Marcelis, J. H.: Interactions between bacteria and iron binding proteins. Veterinary Research Communications 1980, 4: 151–164.

    Google Scholar 

  22. Stark, P. L., Lee, A.: The microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life. Journal of Medical Microbiology 1982, 15: 189–203.

    PubMed  Google Scholar 

  23. De Vos, N., Mevissen-Verhage, E., Harmsen-Van Amerongen, W., Marcelis, J.: A new selective medium for the culture of clostridia from human faeces. European Journal of Clinical Microbiology 1982, 1: 267–271.

    PubMed  Google Scholar 

  24. Drasar, B. S., Jenkins, D. J. A., Cummings, J. H.: The influence of a diet rich in wheat fibre on the human fecal flora. Journal of Medical Microbiology 1976, 9: 423–431.

    PubMed  Google Scholar 

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Mevissen-Verhage, E.A.E., Marcelis, J.H., Harmsen-Van Amerongen, W.C.M. et al. Effect of iron on neonatal gut flora during the first three months of life. Eur. J. Clin. Microbiol. 4, 273–278 (1985). https://doi.org/10.1007/BF02013651

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